关键信息
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基因名
KRT8
- 应用
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别名
Cytokeratin-8 ;CK-8Keratin-8 ;K8Type-II keratin Kb8
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05787
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表达区间
2-483aa
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分子量
69.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Keratin 8 (KRT8) is a type of intermediate filament protein that plays a crucial role in maintaining the structural integrity and mechanical properties of epithelial cells. As a member of the keratin family, KRT8 is primarily expressed in simple epithelia and is often co-expressed with Keratin 18 (KRT18), reflecting its importance in both normal physiology and disease states. Research has increasingly focused on KRT8 due to its potential involvement in various pathological conditions, including liver diseases, certain cancers, and degenerative disorders. The study of recombinant KRT8 protein provides valuable insights into its functional properties and interactions at the molecular level. By utilizing recombinant DNA technology, researchers can produce KRT8 in significant quantities, allowing for extensive in vitro studies to elucidate its role in cellular processes, such as cell differentiation, apoptosis, and response to stress. Furthermore, the pathological implications of KRT8, particularly its aberrant expression patterns observed in tumors, highlight the protein's potential as a biomarker for diagnosis and prognosis in cancer contexts. Overall, KRT8 recombinant protein research enhances our understanding of epithelial biology and opens avenues for novel therapeutic strategies targeting keratin-associated diseases.
