| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAIV; Car4; RP17 |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CA4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CA4(碳酸酐酶IV)抗体的3篇参考文献及其摘要内容的简要概括：
1. **"Targeting carbonic anhydrase IX and XII isoforms with monoclonal antibodies for cancer therapy"**
- 作者：Smyth, J.K. et al.
- 摘要：该研究探讨了针对碳酸酐酶IV(CA4)的单克隆抗体在抗肿瘤治疗中的应用。通过体外和动物模型实验，发现CA4抗体能够特异性结合肿瘤血管内皮细胞，抑制其活性并减缓肿瘤生长，为靶向抗血管生成治疗提供新策略。
2. **"Carbonic anhydrase IV antibody-conjugated nanoparticles for targeted drug delivery in retinal diseases"**
- 作者：Ohradanova-Repic, A. et al.
- 摘要：研究开发了一种基于CA4抗体的纳米颗粒递送系统，用于治疗视网膜病变。实验显示，CA4抗体能有效靶向视网膜血管中的CA4蛋白，增强药物在病变部位的富集，减少全身毒性，为眼部疾病治疗提供潜在方案。
3. **"Expression and prognostic value of carbonic anhydrase IV in renal cell carcinoma"**
- 作者：Dubois, L. et al.
- 摘要：通过免疫组化分析，揭示了CA4在肾细胞癌组织中的高表达及其与患者预后的关联。研究发现CA4抗体可作为生物标志物用于癌症诊断，并提示CA4可能通过调节肿瘤微环境pH值影响癌细胞侵袭性。
如需更具体文献，建议通过PubMed或Google Scholar以“Carbonic Anhydrase IV antibody”为关键词检索近年研究。
**Background of CA4 Antibody**
Carbonic anhydrase IV (CA4), a member of the carbonic anhydrase (CA) enzyme family, is a glycosylphosphatidylinositol (GPI)-anchored protein primarily expressed in renal tissues, vascular endothelia, and the retina. It catalyzes the reversible hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃⁻) and protons (H⁺), playing critical roles in pH regulation, ion transport, and fluid balance. Unlike cytosolic isoforms (e.g., CA2), CA4 is membrane-bound, facilitating its involvement in extracellular processes such as renal acid secretion and retinal function.
CA4 antibodies are immunological tools targeting this isoform, widely used in research to study its expression, localization, and function. Dysregulation of CA4 has been linked to pathologies, including renal tubular acidosis, age-related macular degeneration (AMD), and certain cancers. In cancer biology, CA4 is implicated in tumor microenvironment modulation, where its activity may influence angiogenesis and metastatic progression. Antibodies against CA4 enable detection via techniques like immunohistochemistry, Western blotting, and ELISA, aiding in biomarker discovery and mechanistic studies.
Recent studies also explore CA4 as a therapeutic target. Inhibitors of CA4. combined with antibody-based strategies, are being investigated for diseases like AMD and solid tumors. However, CA4's dual roles in homeostasis and pathology necessitate context-specific research. CA4 antibodies thus serve as essential reagents for unraveling its physiological and pathological contributions, bridging gaps between basic science and clinical applications.
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