Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit

中文名： 人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA Kit

货号: IPD-E04586h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人胰岛素样生长因子结合蛋白1(IGFBP-1)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的IGFBP1含量。IGFBP1即胰岛素样生长因子结合蛋白1，它在调节胰岛素样生长因子生物活性中发挥重要作用。研究机制方面，可通过结合胰岛素样生长因子，影响细胞的增殖、分化和凋亡等过程，在生长发育、代谢调控及肿瘤发生等领域是研究热点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。适用于代谢疾病机制研究、妊娠相关蛋白动态监测（如妊娠期糖尿病模型分析）以及生长发育领域的科研探索，支持科研人员高效开展IGFBP - 1在生理病理状态下的表达水平研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AFBP ELISA Kit; Alpha pregnancy associated endometrial globulin ELISA Kit; Amniotic fluid binding protein ELISA Kit; Binding protein 25 ELISA Kit; Binding protein 26 ELISA Kit; Binding protein 28 ELISA Kit; Growth hormone independent binding protein ELISA Kit; hIGFBP 1 ELISA Kit; hIGFBP1 ELISA Kit; IBP 1 ELISA Kit; IBP-1 ELISA Kit; IBP1 ELISA Kit; IBP1_HUMAN ELISA Kit; IGF binding protein 1 ELISA Kit; IGF BP25 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP 1 ELISA Kit; IGFBP-1 ELISA Kit; IGFBP1 ELISA Kit; Insulin like growth factor binding protein 1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; Placental protein 12 ELISA Kit; PP 12 ELISA Kit; PP12 ELISA Kit
缩写：
IGFBP1
Uniprot No.：
P08833
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
1.56 ng/mL-100 ng/mL
灵敏度：
0.39 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	12.616	12.563	12.347	12.861	12.777	12.563
SD	0.030	0.029	0.028	0.040	0.039	0.041
CV(%)	5.217	5.052	4.912	7.130	6.747	7.143

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

91-99

1:2

Average %

Range %

90-100

1:4

Average %

Range %

95-102

1:8

Average %

Range %

94-101

回收率：

The recovery of human IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-99
EDTA plasma (n=4)	98	95-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
100	2.921	2.950	2.936	2.759
50	2.017	2.073	2.045	1.868
25	1.069	1.052	1.061	0.884
12.5	0.585	0.574	0.580	0.403
6.25	0.356	0.339	0.348	0.171
3.12	0.272	0.261	0.267	0.090
1.56	0.217	0.231	0.224	0.047
0	0.178	0.176	0.177