| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HP1; HP1A; HEL25 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CBX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CBX5(HP1α)抗体的3篇参考文献，涵盖其功能研究和应用场景：
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1. **文献名称**：*"Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain"*
**作者**：Bannister, A.J., et al.
**摘要**：本研究首次揭示了HP1家族蛋白(包括CBX5/HP1α)通过其chromo结构域特异性识别组蛋白H3第9位赖氨酸甲基化(H3K9me3)，并阐明了其在异染色质形成和基因沉默中的关键作用。文中使用CBX5抗体进行免疫荧光染色和染色质免疫沉淀(ChIP)，证实了HP1α在哺乳动物细胞核中的异染色质定位。
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2. **文献名称**：*"HP1α mediates defective chromatin structure and gene expression in senescence and cancer"*
**作者**：Narita, M., et al.
**摘要**：该研究探讨了CBX5/HP1α在细胞衰老和癌症中的双重作用，发现其表达失调导致染色质结构异常和基因表达紊乱。通过CBX5抗体的Western blot和免疫组化分析，作者证明HP1α在多种癌细胞系中表达显著下调，并与患者预后不良相关。
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3. **文献名称**：*"Dynamic interaction of HP1α with mitotic chromosomes regulates kinetochore assembly"*
**作者**：Abe, Y., et al.
**摘要**：本文利用CBX5抗体进行活细胞成像和免疫沉淀实验，揭示了HP1α在有丝分裂期通过与动粒蛋白相互作用调控染色体分离的功能。研究提出HP1α在维持基因组稳定性中具有重要作用，其缺失会导致染色体错误排列和细胞周期停滞。
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以上文献均通过CBX5抗体验证了蛋白表达、定位及功能，涉及表观遗传调控、疾病机制及细胞动力学等领域。
The CBX5 antibody is a crucial tool for studying the Chromobox homolog 5 (CBX5) protein, also known as heterochromatin protein 1 alpha (HP1α). CBX5 is a member of the Polycomb group (PcG) protein family, which plays a central role in epigenetic regulation and chromatin organization. It contains a conserved chromodomain that binds to trimethylated lysine 9 on histone H3 (H3K9me3), a hallmark of transcriptionally repressive heterochromatin. CBX5 facilitates heterochromatin formation, gene silencing, and maintenance of genomic stability by recruiting chromatin-modifying complexes to specific loci. It is involved in diverse cellular processes, including DNA repair, cell cycle regulation, and telomere maintenance.
Researchers use CBX5 antibodies in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its localization, expression levels, and interactions with chromatin. These studies have linked CBX5 dysregulation to cancer progression, aging-related diseases, and developmental disorders, underscoring its biological significance. The antibody's specificity for CBX5 helps distinguish it from other HP1 isoforms (HP1β and HP1γ), enabling precise mechanistic studies. Recent research also explores its role in 3D genome organization and potential as a therapeutic target. Validated CBX5 antibodies are essential for advancing epigenetics and disease-related research, providing insights into chromatin dynamics and gene expression control.
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