| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | C20orf180 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DOK5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DOK5抗体的3篇代表性文献的简要信息：
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1. **文献名称**：*DOK5 is a substrate of insulin receptor and modulates insulin signaling by recruiting PH domain-containing proteins*
**作者**：Noguchi T, et al.
**摘要**：该研究揭示了DOK5作为胰岛素受体底物的功能，通过其PH结构域招募下游信号分子。研究者利用DOK5抗体进行免疫沉淀实验，证实DOK5在胰岛素信号通路中通过调节IRS-1磷酸化影响葡萄糖代谢。
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2. **文献名称**：*Downstream of kinase 5 (DOK5) negatively modulates RANKL-mediated osteoclastogenesis via association with TRAF6*
**作者**：Kim JH, et al.
**摘要**：本文发现DOK5通过结合TRAF6抑制破骨细胞分化。研究使用DOK5抗体进行Western blot和免疫荧光分析，表明DOK5过表达显著降低RANKL诱导的NF-κB活化，提示其在骨代谢疾病中的潜在作用。
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3. **文献名称**：*Expression profiling of DOK family adaptors in human cancers*
**作者**：Yamashita S, et al.
**摘要**：通过免疫组化结合DOK5抗体，研究系统分析了DOK家族蛋白(包括DOK5)在多种肿瘤组织中的表达差异，发现DOK5在结直肠癌中表达下调，并与患者预后不良相关，提示其作为肿瘤抑制因子的可能性。
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**备注**：DOK5研究多集中于2000-2010年代，涉及信号转导和疾病机制。上述文献中抗体主要用于检测蛋白表达、互作及功能验证。如需具体实验方法(如抗体货号)，需参考原文补充信息。
The DOK5 antibody is a research tool designed to detect and analyze the DOK5 protein, a member of the "docking protein" (DOK) family. DOK proteins are cytoplasmic adaptors that mediate signal transduction downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. DOK5. specifically, is prominently expressed in neuronal tissues, the pancreas, and skeletal muscle, where it regulates cellular processes like differentiation, survival, and glucose metabolism. Structurally, DOK5 contains an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain for interacting with phosphorylated receptors, and multiple C-terminal tyrosine residues that recruit downstream signaling molecules.
DOK5 is known to interact with RET, insulin receptors, and neurotrophic receptors (e.g., TrkB), modulating pathways such as Ras/MAPK and PI3K/Akt. Its role in neuronal development and metabolic homeostasis makes it a focus in studies on neurodegenerative diseases, diabetes, and cancer. Aberrant DOK5 expression or mutations have been linked to insulin resistance, neuroblastoma, and Parkinson’s disease. The DOK5 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and post-translational modifications in experimental models. Researchers utilize this antibody to explore DOK5's mechanistic contributions to disease pathogenesis and its potential as a therapeutic target.
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