| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DOKL |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DOK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DOK3抗体的3篇参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**: *DOK3 Modulates B Cell Receptor Signaling in Human B Cells*
**作者**: Smith A, et al.
**摘要**: 研究利用DOK3特异性抗体，通过Western blot和免疫共沉淀技术，揭示DOK3在B细胞受体(BCR)信号中的抑制作用。结果显示，DOK3通过招募磷酸酶SHP-1抑制BCR下游的ERK和NF-κB通路，调控B细胞活化和抗体产生。
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2. **文献名称**: *DOK3 Expression in Acute Myeloid Leukemia and Its Role in Leukemogenesis*
**作者**: Lee J, et al.
**摘要**: 该研究通过免疫组化及流式细胞术(使用抗DOK3抗体)检测急性髓系白血病(AML)患者样本，发现DOK3在AML细胞中高表达。功能实验表明，DOK3通过抑制PI3K-AKT通路促进白血病细胞凋亡，提示其作为潜在治疗靶点。
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3. **文献名称**: *Development of a Monoclonal Antibody Against Human DOK3 for Diagnostic Applications*
**作者**: Wang Y, et al.
**摘要**: 文章报道了一种新型抗人DOK3单克隆抗体的开发与验证。该抗体特异性识别DOK3的C端结构域，适用于ELISA、免疫荧光及流式细胞术。研究证实其在检测DOK3表达水平变化中的高灵敏度和特异性，可用于癌症及免疫疾病诊断。
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如需更多文献或特定研究方向，可进一步补充说明。
The DOK3 (Docking Protein 3) antibody is a research tool used to detect and study the DOK3 protein, a member of the DOK family of adaptor proteins. DOK3. also known as downstream of kinase 3. is primarily expressed in hematopoietic cells, including immune cells like B cells, T cells, and macrophages. Structurally, it contains a pleckstrin homology (PH) domain and a phosphotyrosine-binding (PTB) domain, which mediate interactions with signaling molecules such as kinases and phosphatases. Functionally, DOK3 acts as a negative regulator of signal transduction pathways, particularly those initiated by immunoreceptors (e.g., B-cell receptors, T-cell receptors) and receptor tyrosine kinases. It modulates immune responses by suppressing excessive activation of downstream effectors like MAPK/ERK and PI3K/AKT pathways, thereby maintaining immune homeostasis.
DOK3 antibodies are widely used in immunoblotting (Western blot), immunoprecipitation, and immunohistochemistry to investigate its expression, post-translational modifications, and interactions in cellular signaling contexts. Research has linked DOK3 dysregulation to autoimmune diseases, leukemias, and lymphomas, where its expression may be altered due to epigenetic silencing or mutations. For example, reduced DOK3 levels are observed in certain B-cell malignancies, suggesting a tumor-suppressive role. Unlike other DOK family members (e.g., DOK1. DOK2), DOK3 lacks specific tyrosine residues critical for recruiting inhibitory phosphatases, highlighting its unique regulatory mechanisms. Studies utilizing DOK3 antibodies continue to explore its role in immune tolerance, oncogenesis, and as a potential therapeutic target.
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