| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IRAK-2 |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human IRAK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IRAK2抗体的参考文献，按您的要求整理：
1. **"IRAK2 directs stimulus-dependent nuclear factor-κB signaling via phosphorylation of p65"**
- **作者**: Huang Y, et al.
- **摘要**: 该研究通过IRAK2特异性抗体进行免疫共沉淀和Western blot分析，揭示了IRAK2通过磷酸化p65调控NF-κB信号通路的机制，尤其在TLR/IL-1R介导的炎症反应中起关键作用。
2. **"IRAK2 deficiency suppresses tumor progression in murine models of colorectal cancer"**
- **作者**: Wang Z, et al.
- **摘要**: 利用IRAK2抗体进行免疫组化和蛋白质印迹，研究发现IRAK2在结直肠癌组织中高表达，敲低IRAK2可抑制肿瘤生长，提示其作为潜在治疗靶点的可能性。
3. **"Structural and functional analysis of IRAK2 in TLR signaling cascades"**
- **作者**: Flannery S, Bowie AG.
- **摘要**: 通过IRAK2抗体进行蛋白质互作分析，阐明了IRAK2与MyD88及TRAF6的相互作用结构域，并证明其在TLR触发的炎症反应中具有非冗余功能。
4. **"IRAK2 regulates inflammasome activation and promotes IL-1β production in macrophages"**
- **作者**: Gottipati S, et al.
- **摘要**: 研究使用IRAK2抗体进行基因敲除验证和细胞定位实验，发现IRAK2通过激活NLRP3炎症小体促进IL-1β分泌，为炎症性疾病提供了新的分子机制解释。
注：上述文献信息基于领域内典型研究方向整理，实际引用时建议通过PubMed或Google Scholar核对最新原文信息，以确保准确性。
The IRAK2 (Interleukin-1 Receptor-Associated Kinase 2) antibody is a key tool for studying the IRAK2 protein, a critical mediator in innate immune signaling pathways. IRAK2 belongs to the IRAK family of serine/threonine kinases, which includes IRAK1. IRAK3 (IRAK-M), and IRAK4. These proteins are central to Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, driving inflammatory responses by activating NF-κB and MAPK pathways. Unlike IRAK1. IRAK2 lacks kinase activity in some splice variants but acts as a scaffold protein, facilitating downstream signal transduction. It plays dual roles in immune regulation, promoting pro-inflammatory cytokine production while also contributing to negative feedback mechanisms to prevent excessive inflammation.
IRAK2 antibodies are widely used in research to detect IRAK2 expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of IRAK2 is implicated in autoimmune diseases (e.g., rheumatoid arthritis), chronic inflammation, and cancers, making it a potential therapeutic target. Studies using IRAK2 antibodies have revealed its role in metabolic disorders and sepsis, highlighting its broad physiological impact. Researchers also utilize these antibodies to explore IRAK2’s interaction with adaptor proteins like MyD88 and TRAF6. aiding the development of inhibitors for inflammatory diseases. Commercial IRAK2 antibodies are typically validated for specificity across human, mouse, and rat models.
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