| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | LMN; ADLD; LMN2; LMNB |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human LMNB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于LMNB1抗体的3-4篇参考文献的简要概括：
1. **"Lamin B1 loss in senescent cells promotes nuclear instability"**
- **作者**: Shimi T., et al.
- **摘要**: 研究利用LMNB1抗体分析衰老细胞中核纤层蛋白B1的表达变化，发现其下调导致核膜异常和染色质紊乱，提示LMNB1缺失与衰老相关核结构退化相关。
2. **"Lamin B1 overexpression correlates with poor prognosis in colorectal cancer"**
- **作者**: Zhang Y., et al.
- **摘要**: 通过LMNB1抗体的免疫组化分析，发现结直肠癌组织中LMNB1蛋白过表达与肿瘤转移和患者生存率降低显著相关，表明其可作为潜在预后标志物。
3. **"Antibody-specific validation for lamin B1 in immunofluorescence and Western blotting"**
- **作者**: Dittmer T.A., et al.
- **摘要**: 研究系统评估多种LMNB1抗体的特异性，验证其在细胞分裂期核膜分解检测中的可靠性，为选择高特异性抗体提供实验依据。
4. **"Nuclear lamina dysfunction in neurodegenerative diseases"**
- **作者**: Frost R., et al.
- **摘要**: 使用LMNB1抗体观察阿尔茨海默病患者脑组织，发现核纤层结构异常与神经元退化相关，提示LMNB1异常可能参与神经退行性病变机制。
以上文献聚焦于LMNB1抗体在不同研究场景(如衰老、癌症、方法学验证及神经疾病)中的应用及发现。
LMNB1 (Lamin B1) is a key structural protein component of the nuclear lamina, a meshwork lining the inner nuclear membrane that provides mechanical stability to the nucleus and regulates chromatin organization, gene expression, and DNA replication/repair. Encoded by the *LMNB1* gene, this 66 kDa protein belongs to the type B lamin family, which is ubiquitously expressed in most somatic cells. Unlike A-type lamins, LMNB1 remains expressed throughout the cell cycle and is critical for maintaining nuclear integrity during mitotic processes. Antibodies targeting LMNB1 are widely used in research to investigate nuclear architecture, cellular aging, and diseases linked to nuclear envelope dysfunction.
Studies have shown that LMNB1 downregulation is associated with cellular senescence and aging-related pathologies, while its overexpression is observed in certain cancers, suggesting a role as a potential biomarker. Commercially available LMNB1 antibodies (monoclonal or polyclonal) are commonly validated for applications like Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC). Specificity is often confirmed using knockout (KO) cell lines or tissues. In disease contexts, LMNB1 antibodies help explore laminopathies (e.g., progeria), neurodegenerative disorders, and cancer biology. Recent research also implicates LMNB1 in viral infection responses and chromatin remodeling, expanding its relevance in both basic and translational studies.
×
