| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Car3; CAIII |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CA3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MYLK2抗体的3篇参考文献，按发表时间排序：
1. **文献名称**: "MYLK2 regulates skeletal myoblast differentiation and muscle regeneration"
**作者**: Chen et al. (2018)
**摘要**: 本研究利用特异性MYLK2抗体，通过Western blot和免疫荧光技术，验证了MYLK2在骨骼肌成肌细胞分化中的表达上调，并发现其通过调控肌球蛋白轻链磷酸化促进肌管形成。抗体特异性经siRNA敲低实验证实。
2. **文献名称**: "Characterization of a novel polyclonal antibody against human cardiac MYLK2"
**作者**: Smith et al. (2016)
**摘要**: 文章报道了一种新型多克隆MYLK2抗体的开发与验证。通过免疫组化及心肌组织裂解液检测，证实该抗体可特异性识别人类心脏组织中的MYLK2蛋白，并与肥厚性心肌病患者的MYLK2异常表达相关。
3. **文献名称**: "MYLK2 deficiency disrupts sarcomere organization in zebrafish models"
**作者**: Johnson et al. (2020)
**摘要**: 研究使用商业化MYLK2抗体(ABC公司，货号#AB123)在斑马鱼模型中检测MYLK2蛋白定位。发现MYLK2缺失导致肌节结构紊乱，抗体特异性通过CRISPR-Cas9基因敲除模型的阴性对照实验验证。
注：以上文献信息为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“MYLK2 antibody”、“MYLK2 kinase”或“MYLK2 validation”进一步查找最新研究。
The MYLK2 antibody is a valuable tool for studying myosin light chain kinase 2 (MYLK2), a member of the myosin light chain kinase (MLCK) family. MYLK2. encoded by the *MYLK2* gene in humans (chromosome 20q13.31), is primarily expressed in skeletal and cardiac muscle. It phosphorylates the regulatory light chain of myosin (MLC2), regulating actin-myosin interactions and muscle contraction. Unlike smooth muscle MLCK (MLCK1), MYLK2 is part of the sarcomeric MLCK subfamily and plays a critical role in striated muscle function, particularly in modulating cardiac contractility and skeletal muscle dynamics.
Antibodies targeting MYLK2 enable the detection and quantification of the protein in various experimental settings, including Western blotting, immunohistochemistry, and immunofluorescence. These reagents help investigate MYLK2's expression patterns, subcellular localization, and interactions in physiological and pathological contexts. Dysregulation of MYLK2 has been implicated in cardiomyopathies, skeletal myopathies, and certain cancers, where aberrant muscle contraction or cell motility contributes to disease progression.
Research using MYLK2 antibodies has also explored its role in developmental biology and exercise-induced muscle adaptation. Specificity validation (e.g., knockout controls) is essential due to structural homology among MLCK family members. Recent studies highlight MYLK2's potential as a biomarker or therapeutic target, driving interest in refining antibody-based assays for precision medicine applications.
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