| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAPK14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MAPK14(p38α)抗体的参考文献及其摘要概括：
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1. **文献名称**: *"Selective inhibition of p38α MAPK improves therapeutic targeting in rheumatoid arthritis"*
**作者**: Genovese MC et al.
**摘要**: 该研究使用特异性MAPK14(p38α)抗体验证了其在类风湿关节炎模型中的表达及激活状态，发现抑制p38α可显著减少炎症因子(如TNF-α和IL-6)的释放，为靶向治疗提供依据。
2. **文献名称**: *"p38 MAPK signaling in osteoblast differentiation and bone remodeling"*
**作者**: Greenblatt MB et al.
**摘要**: 通过MAPK14抗体进行免疫印迹和免疫组化分析，揭示了p38α在成骨细胞分化和骨稳态中的关键作用，表明其活性失调可能导致骨质疏松等骨代谢疾病。
3. **文献名称**: *"Role of p38 MAPK in cancer drug resistance: Mechanisms and therapeutic implications"*
**作者**: Wu PK et al.
**摘要**: 利用MAPK14抗体研究p38α在肿瘤细胞中的表达模式，发现其通过调控凋亡和自噬通路介导化疗耐药，提示抑制p38α可能增强传统化疗药物疗效。
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以上文献均通过MAPK14抗体进行机制研究，涵盖炎症、骨代谢及肿瘤领域，可作为相关实验设计的参考。
Mitogen-activated protein kinase 14 (MAPK14), also known as p38α, is a member of the evolutionarily conserved serine/threonine protein kinase family. It plays a central role in cellular responses to stress signals, inflammatory cytokines, and growth factors. MAPK14 is activated through dual phosphorylation of its Thr-Gly-Tyr motif by upstream kinases, triggering downstream targets involved in apoptosis, cell differentiation, and immune regulation. It modulates the activity of transcription factors (e.g., NF-κB, ATF-2) and regulates the production of pro-inflammatory cytokines like TNF-α and IL-1β. Dysregulation of MAPK14 signaling is linked to cancer, neurodegenerative diseases, and autoimmune disorders.
MAPK14 antibodies are essential tools for studying its expression, activation, and subcellular localization. They enable detection of total MAPK14 or its phosphorylated (activated) form in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Phospho-specific antibodies targeting Thr180/Tyr182 residues are widely used to assess kinase activation status in research models of inflammation, oxidative stress, or drug responses. These antibodies also support therapeutic development, as MAPK14 inhibitors are being explored for treating rheumatoid arthritis and other inflammatory conditions. Validation using knockout controls or peptide blocking is critical to ensure antibody specificity due to high homology among p38 isoforms (β, γ, δ).
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