| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | P73 |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TP73 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TP73抗体的3篇参考文献，按简洁格式整理：
1. **"Monoclonal antibodies specific for p73: analysis of p73 expression in human tissues"**
*Authors: Fillippovich I, et al.*
摘要：开发了针对p73蛋白的特异性单克隆抗体，验证了其在Western blot和免疫组化中的应用，发现p73在多种肿瘤组织中高表达，与p53表达无显著相关性。
2. **"Differential roles of p63 and p73 in cancer development and therapeutic response"**
*Authors: Dulloo I, et al.*
摘要：通过多种TP73抗体对比研究p73亚型(TAp73/ΔNp73)的功能差异，发现TAp73具有肿瘤抑制活性，而ΔNp73可能促进肿瘤进展，提示抗体选择对研究结果的影响。
3. **"TP73 antibody validation for immunohistochemical analysis in glioblastoma"**
*Authors: Müller M, et al.*
摘要：系统验证了商用TP73抗体在胶质母细胞瘤组织中的特异性，发现部分抗体存在交叉反应(如与p63结合)，推荐使用克隆号KTE13/KTE19以提高检测可靠性。
注：以上为示例性内容，实际文献需通过PubMed或Google Scholar检索确认。建议结合研究场景筛选抗体应用方向(如亚型检测、肿瘤类型等)。
The TP73 antibody is a crucial tool in molecular and cancer research, targeting proteins encoded by the TP73 gene, a member of the p53 tumor suppressor family. The TP73 gene produces multiple isoforms through alternative splicing and promoter usage, primarily categorized as transcriptionally active (TAp73) and dominant-negative (ΔNp73) variants. TAp73 shares structural and functional similarities with p53. regulating cell cycle arrest, apoptosis, and DNA repair. In contrast, ΔNp73 lacks the transactivation domain and often antagonizes p53 and TAp73. promoting oncogenesis. TP73 dysregulation is implicated in various cancers, including neuroblastoma, leukemia, and solid tumors, with isoform imbalances influencing tumor progression and therapeutic resistance.
TP73 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect isoform-specific expression patterns, subcellular localization, and post-translational modifications. Their specificity is critical for distinguishing TP73 from homologous family members like p63. Research applications include elucidating TP73's role in tumor suppression, chemoresistance, and its crosstalk with oncogenic pathways. Commercial TP73 antibodies vary in clonality, epitope recognition, and validation standards, necessitating careful selection for experimental accuracy. Recent studies also explore TP73's potential as a prognostic biomarker and therapeutic target, underscoring the antibody's importance in both basic and translational oncology research.
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