| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PAK7 |
| WB Predicted band size | 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PAK5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于PAK5抗体的参考文献及其摘要概括：
1. **"Generation and characterization of a monoclonal antibody against human PAK5"**
*作者：Dan, C., et al.*
**摘要**：该研究报道了一种针对人源PAK5蛋白的单克隆抗体的开发与验证。抗体通过重组PAK5片段免疫小鼠制备，经Western blot和免疫荧光验证其特异性，可识别内源性PAK5.并应用于神经母细胞瘤中PAK5表达的检测。
2. **"PAK5 promotes cell migration via aberrant expression of β-catenin in colorectal cancer"**
*作者：Li, X., et al.*
**摘要**：研究利用特异性PAK5抗体探究其在结直肠癌转移中的作用。抗体通过免疫组化验证，显示PAK5高表达与β-catenin异常定位相关，揭示了PAK5通过Wnt信号通路促进肿瘤侵袭的机制。
3. **"A novel polyclonal antibody for detecting PAK5 in human tissues and its application in pancreatic cancer studies"**
*作者：Wang, Y., et al.*
**摘要**：团队开发了一种兔源多克隆抗体，针对PAK5 C端结构域。抗体经ELISA和免疫沉淀验证，成功用于胰腺癌组织切片分析，证实PAK5表达与患者预后不良相关。
4. **"PAK5 kinase activity is required for embryonic neuronal differentiation"**
*作者：Smith, J.L., et al.*
**摘要**：研究通过特异性PAK5抗体(经shRNA敲低验证)探讨其在神经元分化中的功能。抗体用于检测胚胎小鼠脑组织中PAK5的活性形式，发现其激酶活性对神经突生长至关重要。
(注：以上文献为示例，实际引用时需核对真实存在的论文信息。)
The p21-activated kinase 5 (PAK5), a member of the PAK family (Group II PAKs: PAK4-6), is a serine/threonine kinase regulated by Rho GTPases like Cdc42 and Rac. PAK5 plays critical roles in cytoskeletal remodeling, cell survival, and neuronal development by modulating pathways such as MAPK and PI3K/Akt. It is highly expressed in the brain, where it regulates synaptic plasticity and axon guidance, but is also implicated in cancer progression. Overexpression of PAK5 correlates with tumor invasion, metastasis, and therapy resistance in cancers like breast, colorectal, and glioblastoma, often through interactions with β-catenin, Bad, or caspases.
PAK5 antibodies are essential tools for studying its expression, localization, and function. These antibodies, often monoclonal or polyclonal, are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity remains a challenge due to structural homology among PAK family members. High-quality PAK5 antibodies are typically validated using knockout cells or siRNA-mediated silencing to confirm target selectivity. Researchers use these antibodies to explore PAK5's role in disease mechanisms, biomarker discovery, and therapeutic targeting. Recent studies also highlight its potential in neurodegenerative and cardiovascular disorders, expanding its relevance beyond oncology.
×
