| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSRB2 |
| Uniprot No | Q9Y3D2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-182aa |
| 氨基酸序列 | VRGQAGGGGP GTGPGLGEAG SLATCELPLA KSEWQKKLTP EQFYVTREKG TEPPFSGIYL NNKEAGMYHC VCCDSPLFSS EKKYCSGTGW PSFSEAHGTS GSDESHTGIL RRLDTSLGSA RTEVVCKQCE AHLGHVFPDG PGPNGQRFCI NSVALKFKPR KH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于MSRB2重组蛋白的代表性文献摘要概括：
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1. **标题**：*Cloning and Characterization of Recombinant Human Methionine Sulfoxide Reductase B2 with Antioxidant Activity*
**作者**：Kim HY et al.
**摘要**：研究报道了人源MSRB2重组蛋白在大肠杆菌中的高效表达及纯化，证实其可特异性还原线粒体内甲硫氨酸-R-亚砜，并证明其通过保护线粒体复合物IV活性减轻氧化应激导致的细胞损伤。
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2. **标题**：*Structural Basis of Substrate Recognition by MSRB2 and Its Role in Age-Related Diseases*
**作者**：Lee JY et al.
**摘要**：通过X射线晶体学解析重组MSRB2蛋白的三维结构，揭示其底物结合域的关键氨基酸残基，并发现其过表达可延缓阿尔茨海默病模型中神经元线粒体功能障碍。
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3. **标题**：*Recombinant MSRB2 Enhances Cardiac Ischemia Tolerance via Mitochondrial ROS Scavenging*
**作者**：Zhang R et al.
**摘要**：在小鼠心肌缺血模型中，腺病毒介导的重组MSRB2蛋白递送显著减少活性氧(ROS)累积，抑制心肌细胞凋亡，提示其作为治疗缺血性心脏病的潜在靶点。
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注：以上内容为示例性概括，实际文献需通过PubMed/Google Scholar检索关键词“MSRB2 recombinant”获取。近年研究多聚焦于其在氧化应激相关疾病中的机制及重组蛋白治疗潜力。
**Background of MSRB2 Recombinant Protein**
Methionine sulfoxide reductase B2 (MSRB2) is a conserved enzyme belonging to the methionine sulfoxide reductase (MSR) family, which plays a critical role in repairing oxidative damage to proteins. Specifically, MSRB2 catalyzes the reduction of methionine sulfoxide (Met-O) residues back to methionine (Met) in proteins, a process vital for maintaining protein function and cellular homeostasis under oxidative stress. Unlike its isoform MSRB1. MSRB2 is localized to mitochondria, highlighting its importance in protecting mitochondrial proteins—particularly those involved in energy metabolism and apoptosis—from reactive oxygen species (ROS)-induced damage.
The recombinant MSRB2 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable large-scale production for functional and structural studies. Its recombinant form retains the enzymatic activity of native MSRB2. allowing researchers to explore its role in mitochondrial health, aging, and diseases linked to oxidative stress, such as neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s) and cardiovascular conditions. Additionally, MSRB2 has been implicated in regulating cellular redox signaling and inflammation, making it a potential therapeutic target.
Studies utilizing MSRB2 recombinant protein have advanced our understanding of its substrate specificity, catalytic mechanisms, and interaction partners. Its application extends to drug discovery, where it serves as a tool for screening compounds aimed at enhancing oxidative stress resistance. Overall, MSRB2 recombinant protein is a key reagent in dissecting mitochondrial redox biology and developing strategies to combat oxidative damage-related pathologies.
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