| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS25 |
| Uniprot No | P82663 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-173aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMPMKGRFPIRRTLQYLSQGNVVFKDSV KVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLR FYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPA NFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPS25重组蛋白的假设参考文献示例(注：文献为假设模拟，实际文献需通过学术数据库查询)：
1. **文献名称**：*MRPS25 Recombinant Protein Facilitates Mitochondrial Ribosome Assembly in Mammalian Cells*
**作者**：Lee, J. et al.
**摘要**：研究利用重组MRPS25蛋白，通过体外线粒体核糖体重组实验，揭示了MRPS25在小亚基组装中的关键作用，并发现其缺失会导致氧化磷酸化功能受损。
2. **文献名称**：*Structural and Functional Analysis of MRPS25 in Cancer Cell Metabolism*
**作者**：Zhang, Y. et al.
**摘要**：通过表达纯化重组MRPS25蛋白，结合X射线晶体学解析其结构，发现其与肺癌细胞中线粒体翻译活性的相关性，并验证其作为潜在治疗靶点的可能性。
3. **文献名称**：*Recombinant MRPS25 Expression System Optimized for High-Yield Production in E. coli*
**作者**：Smith, R. et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于生产可溶性的重组MRPS25蛋白，优化后的蛋白适用于抗体开发及蛋白质相互作用研究。
4. **文献名称**：*MRPS25 Deficiency Disrupts Mitochondrial Protein Synthesis and Promotes Neurodegeneration*
**作者**：Wang, H. et al.
**摘要**：利用重组MRPS25蛋白恢复突变细胞模型，证明其在维持线粒体mRNA翻译中的必要性，并关联MRPS25突变与神经退行性疾病的发生机制。
---
**注意**：以上文献为示例性质，实际研究需通过PubMed、Web of Science等平台检索关键词(如“MRPS25 recombinant protein”或“mitochondrial ribosome protein S25”)获取真实文献。
MRPS25 (Mitochondrial Ribosomal Protein S25) is a key component of the mitochondrial ribosome small subunit (28S), essential for mitochondrial protein synthesis. Encoded by nuclear DNA and imported into mitochondria, MRPS25 plays a critical role in assembling and stabilizing the ribosome structure, facilitating the translation of mitochondrial DNA-encoded genes. These genes produce subunits of oxidative phosphorylation (OXPHOS) complexes, which are vital for ATP production and cellular energy metabolism.
Dysregulation of MRPS25 is linked to mitochondrial dysfunction, implicated in diseases such as cancer, neurodegenerative disorders (e.g., Parkinson’s), and metabolic syndromes. For instance, reduced MRPS25 expression disrupts OXPHOS, leading to impaired energy production and increased oxidative stress, contributing to disease progression. In cancer, altered mitochondrial ribosome function may promote tumor survival under metabolic stress.
Recombinant MRPS25 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) with affinity tags (e.g., His-tag) for purification. This engineered protein enables functional studies, including ribosome assembly assays, protein-protein interaction analyses, and investigations into mitochondrial translation mechanisms. It also serves as an antigen for antibody development or a standard in diagnostic assays. Researchers utilize recombinant MRPS25 to explore its role in disease pathways and screen therapeutic compounds targeting mitochondrial dysfunction. Structural studies using recombinant protein further elucidate its molecular interactions within the ribosome, advancing our understanding of mitochondrial biology and associated pathologies.
×
