| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS28 |
| Uniprot No | Q9Y2Q9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 72-187aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSGSPKNVE SFASMLRHSP LTQMGPAKDK LVIGRIFHIV ENDLYIDFGG KFHCVCRRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT DTTVLEANAV LLGIQESKDS RSKEEHHEK |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPS28重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Structural and functional analysis of MRPS28 in mitochondrial ribosome assembly*
**作者**：Li Y, et al.
**摘要**：本研究通过重组表达人源MRPS28蛋白，结合冷冻电镜技术解析其在线粒体核糖体小亚基中的结构定位，揭示了MRPS28在核糖体组装及稳定性维持中的关键作用，并发现其C端结构域对结合mRNA至关重要。
2. **文献名称**：*MRPS28 mutations impair mitochondrial translation and cause early-onset neurological disorders*
**作者**：Wang X, et al.
**摘要**：通过重组MRPS28蛋白的体外功能实验，发现其特定突变(如p.Arg54Cys)会导致线粒体翻译缺陷，引发患者能量代谢障碍，研究为MRPS28相关线粒体疾病的分子机制提供了实验证据。
3. **文献名称**：*Recombinant MRPS28 expression in E. coli: A strategy for studying mitochondrial protein synthesis defects*
**作者**：Zhang R, et al.
**摘要**：报道了一种高效表达和纯化重组MRPS28蛋白的大肠杆菌系统，通过体外重建实验证明重组蛋白可恢复缺陷型线粒体的翻译活性，为相关疾病模型构建提供了工具。
(注：以上文献为示例，实际引用需根据具体研究补充完整信息。)
**Background of MRPS28 Recombinant Protein**
MRPS28 (Mitochondrial Ribosomal Protein S28) is a key component of the mitochondrial ribosome’s small subunit (28S), playing a critical role in mitochondrial protein synthesis. Mitochondria, known as the cellular powerhouses, rely on their own translation machinery to produce essential subunits of the oxidative phosphorylation (OXPHOS) complexes, which are vital for ATP production. MRPS28 contributes to the structural integrity and function of the mitochondrial ribosome, ensuring accurate translation of mitochondrial DNA (mtDNA)-encoded mRNAs.
The recombinant MRPS28 protein is engineered using biotechnological approaches, typically expressed in *E. coli* or mammalian cell systems, followed by purification to homogeneity. This allows researchers to study its molecular interactions, structural features, and functional mechanisms in vitro. Recombinant MRPS28 is often tagged (e.g., His-tag, FLAG-tag) to facilitate detection and purification.
Studies on MRPS28 are crucial for understanding mitochondrial disorders linked to ribosomal dysfunction, such as encephalopathies, cardiomyopathies, and metabolic syndromes. Mutations in mitochondrial ribosomal proteins, including MRPS28. have been associated with impaired OXPHOS activity, leading to cellular energy deficits and organ dysfunction. Additionally, MRPS28 has been implicated in cancer progression, as altered mitochondrial translation may support tumor cell proliferation under stress.
The availability of recombinant MRPS28 enables functional assays, antibody production, and structural studies (e.g., cryo-EM) to explore ribosome assembly and mitochondrial translation regulation. It also serves as a tool for drug screening aimed at modulating mitochondrial function in diseases. Challenges remain in mimicking post-translational modifications and ensuring proper folding of the recombinant protein for accurate biological activity.
Overall, MRPS28 recombinant protein is a valuable resource for advancing mitochondrial biology research and therapeutic development.
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