| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS2 |
| Uniprot No | Q9Y399 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-296aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMATSSAALPRILGAGARAPSRWLGFLG KATPRPARPSRRTLGSATALMIRESEDSTDFNDKILNEPLKHSDFFNVKE LFSVRSLFDARVHLGHKAGCRHRFMEPYIFGSRLDHDIIDLEQTATHLQL ALNFTAHMAYRKGIILFISRNRQFSYLIENMARDCGEYAHTRYFRGGMLT NARLLFGPTVRLPDLIIFLHTLNNIFEPHVAVRDAAKMNIPTVGIVDTNC NPCLITYPVPGNDDSPLAVHLYCRLFQTAITRAKEKRQQVEALYRLQGQK EPGDQGPAHPPGADMSHSL |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPS2重组蛋白的3篇文献摘要示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索获取)：
---
1. **文献名称**: "Recombinant expression and functional characterization of MRPS2 in mitochondrial translation"
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功制备了重组人源MRPS2蛋白，并验证其在线粒体核糖体组装中的关键作用。通过体外翻译实验发现，MRPS2缺失会导致线粒体蛋白质合成效率显著下降，提示其在能量代谢疾病中的潜在病理机制。
---
2. **文献名称**: "Structural insights into MRPS2 using cryo-EM and recombinant protein technology"
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜技术结合重组MRPS2蛋白的表达纯化，解析了线粒体核糖体小亚基复合物的高分辨率结构。研究表明MRPS2与相邻亚基(如MRPS5)形成关键界面，为靶向线粒体翻译的药物设计提供结构基础。
---
3. **文献名称**: "MRPS2 overexpression promotes cancer cell proliferation via mTOR pathway activation"
**作者**: Wang Y, et al.
**摘要**: 通过重组MRPS2蛋白的体外功能实验，发现其过表达可激活mTOR信号通路，促进肿瘤细胞增殖。该研究揭示了MRPS2作为癌症治疗靶点的潜力，并开发了基于重组蛋白的高通量抑制剂筛选平台。
---
如需具体文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词："MRPS2 recombinant" 或 "MRPS2 mitochondrial ribosome"。
**Background of MRPS2 Recombinant Protein**
Mitochondrial Ribosomal Protein S2 (MRPS2) is a critical component of the mitochondrial ribosome, specifically the 28S small subunit (mt-SSU), which plays an essential role in mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translational machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which are vital for cellular respiration and ATP production. Unlike cytosolic ribosomes, mitochondrial ribosomes are structurally and functionally distinct, with MRPS2 contributing to their unique architecture and function.
The MRPS2 gene is nuclear-encoded, and the protein is imported into mitochondria post-translationally. It facilitates the proper assembly and stability of the mt-SSU, ensuring accurate translation of mitochondrial DNA (mtDNA)-encoded mRNAs. Mutations or dysregulation in MRPS2 have been linked to mitochondrial disorders, such as encephalopathies, cardiomyopathies, and metabolic deficiencies, highlighting its importance in maintaining mitochondrial health.
Recombinant MRPS2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable detailed biochemical and functional studies. This engineered protein retains the native structure and activity of endogenous MRPS2. allowing researchers to investigate its interactions with ribosomal components, its role in mitochondrial translation, and its implications in disease mechanisms. Applications include in vitro reconstitution of ribosomal subunits, drug screening for mitochondrial disorders, and structural studies (e.g., cryo-EM) to map ribosome assembly pathways.
Quality-controlled recombinant MRPS2 is essential for reproducibility in research, often verified via SDS-PAGE, mass spectrometry, and functional assays. Its availability accelerates the exploration of mitochondrial biology and the development of targeted therapies for mitochondrial diseases.
×
