| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL28 |
| Uniprot No | Q13084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-256aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL28重组蛋白的参考文献示例(内容基于学术背景知识整理，具体文献需通过数据库验证)：
1. **《Mitochondrial Ribosomal Protein L28 Modulates Mammalian Cell Energy Metabolism》**
- 作者：Smith A, et al.
- 摘要：研究MRPL28在小鼠细胞线粒体核糖体组装中的作用，发现其缺失导致氧化磷酸化功能受损，重组蛋白表达可部分恢复能量代谢异常。
2. **《Structural Insights into the Role of MRPL28 in Mitochondrial Translation》**
- 作者：Chen L, et al.
- 摘要：通过重组MRPL28蛋白的晶体结构解析，揭示其与线粒体rRNA的相互作用，阐明其在蛋白质翻译起始中的分子机制。
3. **《MRPL28 Gene Mutation Linked to Human Cardiomyopathy: Functional Rescue by Recombinant Expression》**
- 作者：Wang Y, et al.
- 摘要：报道MRPL28突变与扩张型心肌病的关联，并在体外实验中证明重组MRPL28蛋白可修复患者细胞中线粒体呼吸链复合体活性。
4. **《Heterologous Expression and Purification of Human MRPL28 in E. coli for Antibody Production》**
- 作者：Kimura T, et al.
- 摘要：描述利用大肠杆菌系统高效表达可溶性MRPL28重组蛋白的优化方法，成功制备用于特异性抗体开发的高纯度抗原。
**建议**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台以“MRPL28 recombinant protein”“mitochondrial ribosome L28”等关键词检索，并重点关注涉及重组蛋白制备、功能研究或疾病模型的论文。
MRPL28 (Mitochondrial Ribosomal Protein L28) is a key component of the mitochondrial ribosome large subunit, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translational machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP production. The mitochondrial ribosome, distinct from its cytosolic counterpart, comprises numerous mitochondrion-specific proteins like MRPL28. reflecting evolutionary adaptations for mitochondrial function. MRPL28 plays a structural and functional role in ribosome assembly and stability, ensuring accurate translation of mitochondrial DNA (mtDNA)-encoded mRNAs.
Recombinant MRPL28 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for research. Its recombinant form enables detailed studies of mitochondrial ribosome biogenesis, protein-protein interactions, and mechanisms underlying mitochondrial translation defects. Dysregulation of MRPL28 has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases, as impaired OXPHOS contributes to cellular energy deficits and metabolic stress. Researchers employ recombinant MRPL28 to investigate disease pathogenesis, screen therapeutic compounds, or validate structural models of mitochondrial ribosomes. Additionally, it serves as an antigen for antibody development in diagnostic assays. The availability of recombinant MRPL28 advances understanding of mitochondrial biology and its implications in human health, offering tools to explore therapeutic strategies targeting mitochondrial dysfunction.
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