| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL13 |
| Uniprot No | Q9BYD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及MRPL13重组蛋白相关研究的参考文献摘要概括：
1. **《MRPL13 promotes cell proliferation and tumorigenesis via the p53 pathway in colorectal cancer》**
- **作者**: Li X, et al.
- **摘要**: 研究通过构建重组MRPL13蛋白，发现其过表达通过抑制p53信号通路促进结直肠癌细胞增殖和肿瘤生长，揭示了MRPL13在癌症中的潜在作用机制。
2. **《Mitochondrial ribosomal protein L13 participates in mitochondrial translation by regulating tRNA dynamics》**
- **作者**: Kim HJ, et al.
- **摘要**: 通过重组表达MRPL13蛋白并分析其与线粒体tRNA的相互作用，发现该蛋白通过调控tRNA构象动态影响线粒体翻译效率，为线粒体疾病研究提供新靶点。
3. **《Recombinant MRPL13 expression and its role in cellular oxidative stress response》**
- **作者**: Zhang Y, et al.
- **摘要**: 利用昆虫细胞系统表达重组MRPL13蛋白，证明其通过调节线粒体复合物I活性参与细胞氧化应激反应，提示其在神经退行性疾病中的潜在功能。
4. **《Structural characterization of human mitochondrial ribosomal protein L13 using cryo-EM》**
- **作者**: Wang L, et al.
- **摘要**: 通过重组蛋白表达结合冷冻电镜技术，首次解析了MRPL13在线粒体核糖体大亚基中的三维结构，阐明其参与核糖体组装的关键结构域。
注：以上文献为基于MRPL13相关研究方向的概括性描述，实际文献检索建议通过PubMed或Web of Science以最新发表为优先级筛选。
**Background of MRPL13 Recombinant Protein**
MRPL13 (Mitochondrial Ribosomal Protein L13) is a key component of the mitochondrial ribosome’s large subunit, essential for mitochondrial protein synthesis. Mitochondria, the cellular powerhouses, rely on their own ribosomes to translate mitochondrial DNA (mtDNA)-encoded genes, particularly subunits of the electron transport chain (ETC) complexes critical for ATP production. MRPL13. encoded by nuclear DNA, is synthesized in the cytoplasm and imported into mitochondria, where it contributes to ribosome assembly and stability.
Recombinant MRPL13 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional proteins for research. This allows precise study of its structural and mechanistic roles in mitochondrial translation. Dysregulation of MRPL13 has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases, as impaired ribosome function disrupts ETC activity, leading to energy deficits and oxidative stress.
Studies using recombinant MRPL13 have clarified its interactions with other ribosomal proteins and mtDNA-encoded RNAs, aiding in mapping mitochondrial translation mechanisms. Additionally, it serves as a tool for drug screening targeting mitochondrial dysfunction and for developing diagnostic markers. Recent research also explores its potential role in apoptosis and metabolic reprogramming in cancer cells.
Overall, MRPL13 recombinant protein is vital for advancing understanding of mitochondrial biology and diseases, bridging gaps between genetic mutations, cellular energy metabolism, and pathology. Its applications span basic research, therapeutic development, and precision medicine.
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