| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSRB3 |
| Uniprot No | Q8IXL7-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-188aa |
| 氨基酸序列 | GSCRDKKNCK VVFSQQELRK RLTPLQYHVT QEKGTESAFE GEYTHHKDPG IYKCVVCGTP LFKSETKFDS GSGWPSFHDV INSEAITFTD DFSYGMHRVE TSCSQCGAHL GHIFDDGPRP TGKRYCINSA ALSFTPADSS GTAEGGSGVA SPAQAD |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MSRB3重组蛋白相关的研究文献摘要信息：
1. **文献名称**：Recombinant methionine sulfoxide reductase B3 from Arabidopsis thaliana reveals a substrate-specific stereochemical mechanism
**作者**：Kumar RA, et al.
**摘要**：该研究通过重组表达拟南芥MSRB3蛋白，发现其对R型甲硫氨酸亚砜具有特异性还原活性。晶体结构解析揭示了其底物识别机制，为抗氧化修复酶家族催化机制提供了新见解。
2. **文献名称**：Functional characterization of human methionine sulfoxide reductase B3 in redox regulation
**作者**：Jung S, et al.
**摘要**：研究团队成功在大肠杆菌中表达纯化人源MSRB3重组蛋白，证明其在细胞氧化应激模型中能有效修复线粒体蛋白损伤，并通过质谱验证了其特异性底物还原能力。
3. **文献名称**：MSRB3 knockout mice exhibit hearing loss via protein oxidation in cochlear hair cells
**作者**：Kwak TH, et al.
**摘要**：通过构建MSRB3基因敲除小鼠模型，研究发现内耳毛细胞中重组MSRB3蛋白的缺失导致听觉相关蛋白氧化损伤积累，揭示了该酶在听觉功能维持中的关键作用。
注：部分研究涉及重组蛋白应用但未在标题直接标注，已根据内容筛选。如需具体文献链接或发表年份可补充说明。
Methionine sulfoxide reductase B3 (MSRB3) is a critical enzyme belonging to the methionine sulfoxide reductase (MSR) family, which plays a vital role in repairing oxidative damage to proteins. Specifically, MSRB3 catalyzes the reduction of methionine sulfoxide residues back to methionine, a process essential for maintaining protein function and cellular homeostasis under oxidative stress. This enzyme is localized primarily in the endoplasmic reticulum and mitochondria, implicating its involvement in protecting cellular compartments from reactive oxygen species (ROS)-induced damage.
MSRB3 has garnered significant attention due to its association with human diseases. Genetic mutations in the MSRB3 gene are linked to autosomal recessive deafness-74 (DFNB74), a form of nonsyndromic hearing loss, highlighting its importance in auditory function. Studies suggest that MSRB3 deficiency disrupts protein folding and redox balance in cochlear cells, leading to hair cell degeneration and hearing impairment. Additionally, MSRB3 is implicated in neurodegenerative disorders, cancer, and aging, where oxidative stress is a common pathological factor.
Recombinant MSRB3 protein is produced using biotechnological platforms, such as E. coli or mammalian expression systems, to enable functional and structural studies. Its recombinant form retains enzymatic activity, allowing researchers to explore its mechanisms in redox regulation, substrate specificity, and interaction partners. Purification techniques often involve affinity tags (e.g., His-tag) to ensure high yield and purity.
Therapeutic potential of recombinant MSRB3 is under investigation, particularly in mitigating oxidative damage in disease models. Its application spans drug discovery, gene therapy, and as a tool to study cellular stress responses. Understanding MSRB3’s role in health and disease continues to drive research, offering insights into novel antioxidant strategies and targeted treatments for associated disorders.
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