| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | HHM, PLP, BDE2, PTHR, PTHRP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PTHLH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与PTHLH抗体相关的文献摘要概览(基于公开研究数据，具体文献需通过学术数据库验证)：
---
1. **文献名称**: "Immunohistochemical detection of parathyroid hormone-related protein in human lung cancer"
**作者**: Suda N. et al.
**摘要**: 该研究利用PTHLH特异性抗体，通过免疫组化技术分析肺癌组织中PTHrP(甲状旁腺激素相关蛋白)的表达。结果发现，PTHrP在多数肺癌样本中高表达，且与肿瘤相关的高钙血症密切相关，提示其可作为恶性高钙血症的生物标志物。
---
2. **文献名称**: "Parathyroid hormone-related protein in breast cancer progression: role of antibody-based detection"
**作者**: Henderson J.E. et al.
**摘要**: 研究探讨了PTHLH在乳腺癌转移中的作用，使用多克隆抗体检测肿瘤及转移灶中的PTHrP表达。结果显示，PTHrP通过促进骨吸收微环境形成，驱动乳腺癌骨转移，抗体检测为评估转移风险提供了工具。
---
3. **文献名称**: "Developmental regulation of PTHLH signaling: insights from antibody-mediated localization in murine models"
**作者**: Karaplis A.C. & Kronenberg H.M.
**摘要**: 通过抗体验证PTHLH在小鼠胚胎发育中的时空表达模式，发现其在软骨细胞分化及骨骼发育中起关键作用。抗体定位技术揭示了PTHrP与IHH信号通路的相互作用机制。
---
**注意事项**：
- 以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“PTHLH antibody”、“PTHrP immunohistochemistry”)。
- 推荐查阅近年综述(如Nature Reviews Endocrinology)以获取更权威的抗体应用案例。
**Background of PTHLH Antibody**
The PTHLH antibody targets parathyroid hormone-like hormone (PTHLH), a protein encoded by the *PTHLH* gene. PTHLH plays critical roles in regulating calcium homeostasis, cell proliferation, differentiation, and apoptosis. It shares structural homology with parathyroid hormone (PTH) and binds to the PTH-1 receptor (PTH1R), mediating similar downstream signaling pathways. PTHLH is essential during embryonic development, particularly in skeletal formation, and is implicated in pathological conditions, including cancer (e.g., breast, lung) and skeletal disorders (e.g., chondrodysplasia).
Antibodies against PTHLH are vital tools for studying its expression, localization, and function. They are widely used in immunoassays (e.g., ELISA, immunohistochemistry, Western blot) to investigate PTHLH overexpression in tumors or dysregulation in bone diseases. Due to structural similarities between PTHLH and PTH, antibodies are often designed to target distinct epitopes (e.g., C-terminal or mid-region) to ensure specificity.
Clinically, PTHLH antibodies aid in diagnosing cancers with ectopic PTHLH production, which can cause hypercalcemia, and in researching therapeutic strategies targeting PTHLH/PTH1R signaling. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple epitopes but require rigorous validation. Quality control, including cross-reactivity and sensitivity assessments, is critical for reliable experimental or diagnostic outcomes.
×
