| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | D22S749 |
| WB Predicted band size | 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PVALB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PVALB(Parvalbumin)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **"Characterization of a novel monoclonal antibody against parvalbumin with superior species cross-reactivity"**
*作者：Schmidt et al.*
**摘要**：研究团队开发了一种新型单克隆PVALB抗体，通过免疫印迹和免疫组化验证其在人类、小鼠和大鼠脑组织中的高特异性。抗体能清晰标记皮质和海马区的PVALB阳性中间神经元，并适用于冷冻和石蜡包埋样本。
2. **"Parvalbumin antibody validation for multiplex fluorescence imaging in neurodegenerative models"**
*作者：Li & Gomez-Cruz*
**摘要**：本文系统评估了多种商业PVALB抗体在神经退行性疾病模型中的应用。通过比较免疫荧光染色效果，发现某些抗体在小鼠和猕猴脑切片中表现稳定，但在阿尔茨海默病模型中可能出现非特异性结合，强调抗体验证的重要性。
3. **"Differential expression of parvalbumin in autism spectrum disorder: Insights from a validated polyclonal antibody approach"**
*作者：Park et al.*
**摘要**：研究利用一种经过验证的多克隆PVALB抗体，发现自闭症患者前额叶皮层中PVALB阳性神经元密度显著降低。实验结合流式细胞术和共聚焦成像，证实抗体的可靠性及在病理研究中的潜在价值。
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这些文献涵盖抗体开发、验证及疾病研究应用，可为实验设计提供参考。如需具体文献，建议通过PubMed或Google Scholar搜索关键词“parvalbumin antibody validation”或“PVALB immunohistochemistry”获取最新研究。
Parvalbumin (PVALB) is a calcium-binding protein belonging to the EF-hand family, primarily expressed in fast-twitching muscle fibers and a subset of GABAergic neurons in the central nervous system. With a molecular weight of approximately 12 kDa, PVALB regulates intracellular calcium dynamics by buffering and transporting Ca²⁺ ions, influencing muscle contraction-relaxation cycles and neuronal excitability. In the brain, PVALB is a marker for fast-spiking interneurons critical for maintaining network oscillations and synaptic plasticity, particularly in regions like the hippocampus, cortex, and cerebellum.
PVALB antibodies are essential tools for identifying and studying these specialized cells in neurological research. They enable visualization of PVALB⁺ neurons via immunohistochemistry, aiding investigations into disorders such as epilepsy, schizophrenia, and autism, where PVALB expression is often altered. In muscular studies, these antibodies help characterize skeletal muscle fiber subtypes and assess pathologies like muscular dystrophy. Additionally, PVALB antibodies are used in Western blotting and flow cytometry to quantify protein levels, providing insights into calcium signaling mechanisms. Their specificity and reliability make them invaluable for understanding both neural circuitry and muscle physiology, bridging molecular biology with functional studies in health and disease.
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