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Mouse Macrophage Migration Inhibitory Factor,MIF ELISA Kit

  • 中文名： 小鼠巨噬细胞移动抑制因子(MIF)ELISA Kit
货号: IPD-E07292m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MIF含量。MIF即巨噬细胞移动抑制因子，是一种多功能细胞因子。它在炎症、免疫反应及肿瘤发生发展等过程中扮演重要角色。研究机制上，MIF可与多种受体结合，激活下游信号通路，调控细胞的增殖、凋亡和炎症因子释放等，是极具潜力的治疗靶点。试剂盒检测范围为125 pg/ml- 8000 pg/ml，灵敏度为31.25 pg/ml。适用于基础医学研究中炎症机制探索、免疫调控实验验证，以及心血管疾病、代谢综合征等动物模型的生物标志物分析，可广泛用于药物开发过程中抗炎化合物或免疫调节剂的体外效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    MifMacrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Delayed early response protein 6 ELISA Kit; DER6 ELISA Kit; Glycosylation-inhibiting factor ELISA Kit; GIF ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
  • 缩写：
    MIF
  • Uniprot No.：
    P34884
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/ml - 8000 pg/ml
  • 灵敏度：
    31.25 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    100


    Range %

    95-105


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    97


    Range %

    92-107


    1:8

    Average %

    92


    Range %

    86-98


  • 回收率：

    The recovery of mouse MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    99-110


    EDTA plasma (n=4)

    97

    90-106


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    8000

    2.326

    2.434

    2.380

    2.212


    4000

    1.818

    1.767

    1.793

    1.625


    2000

    1.243

    1.228

    1.236

    1.068


    1000

    0.759

    0.729

    0.744

    0.576


    500

    0.417

    0.421

    0.419

    0.251


    250

    0.327

    0.333

    0.330

    0.162


    125

    0.263

    0.274

    0.269

    0.101


    0

    0.171

    0.165

    0.168




  • 货期：
    3-5 working days

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