Mouse Neprilysin(MME) ELISA kit

中文名： 小鼠脑啡肽酶(MME)ELISA kit

货号: IPD-EL014653MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠脑啡肽酶(MME)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的MME含量。MME即膜金属内肽酶，在众多生理过程中发挥重要作用。其在细胞信号转导等方面有研究价值，参与调节多种生物活性肽的功能。它作为药物靶点，在高血压、心血管疾病等治疗领域被深入研究，有望为相关疾病治疗带来新策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。适用于小鼠模型的基础研究，例如阿尔茨海默病、神经性疼痛等疾病机制探索，或药理学实验中MME表达水平变化的动态监测。该产品为科研领域提供可靠工具，助力神经生物学、病理生理学等相关研究中对MME功能的深入解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
MmeNeprilysin ELISA Kit; EC 3.4.24.11 ELISA Kit; Atriopeptidase ELISA Kit; Enkephalinase ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; NEP ELISA Kit; Neutral endopeptidase ELISA Kit; Skin fibroblast elastase ELISA Kit; SFE ELISA Kit; CD antigen CD10 ELISA Kit
缩写：
MME
Uniprot No.：
Q61391
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma,tissue homogenates
检测范围：
0.156 ng/mL-10 ng/mL
灵敏度：
0.039 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)1.1911.2401.2251.2381.2491.202SD0.0430.0370.0450.0490.0550.052CV(%)5.1374.2825.2575.6786.3296.168

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MME in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

94-103

1:2

Average %

Range %

80-90

1:4

Average %

100

Range %

97-103

1:8

Average %

Range %

83-89

回收率：

The recovery of mouse MME spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	102	97-106
EDTA plasma (n=4)	89	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
10	2.577	2.601	2.589	2.458
5	2.150	2.064	2.107	1.976
2.5	1.424	1.436	1.430	1.299
1.25	0.860	0.835	0.848	0.717
0.625	0.557	0.538	0.548	0.417
0.312	0.365	0.371	0.368	0.237
0.156	0.259	0.238	0.249	0.118
0	0.131	0.130	0.131