首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠纤维蛋白溶解酶原(PLG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLG含量。PLG即纤溶酶原，是纤溶系统的关键成分。其激活后转化为纤溶酶，参与溶解血栓。研究机制主要围绕调控PLG激活过程，如激活剂和抑制剂的作用。对PLG深入研究有助于理解血栓性疾病病理，为开发抗栓和溶栓药物提供方向。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，灵敏度为15.63 ng/mL。适用于心血管疾病模型、血栓形成机制、炎症相关研究及肿瘤微环境分析等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
缩写：PLG
Uniprot No.：P20918
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.63 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 98 | |||||
| Range % | 92-103 | ||||||
| 1:200 | Average % | 97 | |||||
| Range % | 93-102 | ||||||
| 1:400 | Average % | 104 | |||||
| Range % | 98-108 | ||||||
| 1:800 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
回收率：
| The recovery of mouse PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-93 | |||||
| EDTA plasma (n=4) | 94 | 88-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 40000.0980.0990.099 20000.1250.1320.129 10000.1790.1840.182 5000.2900.3080.299 2500.4620.4750.469 1250.7580.7880.773 62.51.0151.1031.059 02.0022.1102.056 | |||||||
货期：3-5 working days
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