| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 103 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ACTN3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACTN3抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*"A gene for speed: the emerging role of α-actinin-3 in muscle metabolism"*
**作者**：Yang, N., MacArthur, D.G., Gulbin, J.P. et al.
**摘要**：该研究探讨了ACTN3基因型(R577X多态性)与运动表现的关系，利用ACTN3特异性抗体进行Western blot分析，发现α-actinin-3蛋白在快肌纤维中的表达缺失与爆发力运动能力下降相关。
2. **文献名称**：*"Differential expression of skeletal muscle α-actinins in myopathies and regeneration"*
**作者**：North, K.N., Laing, N.G., Beggs, A.H.
**摘要**：通过免疫组化和免疫印迹技术，研究者使用ACTN3抗体比较了正常与病变肌肉组织中α-actinin-3的表达差异，揭示了其在肌肉再生中的潜在作用及与某些肌病的关联。
3. **文献名称**：*"ACTN3 genotype influences muscle performance through the regulation of metabolic pathways"*
**作者**：Eynon, N., Ruiz, J.R., Meckel, Y. et al.
**摘要**：该研究结合基因分型和蛋白质检测(使用ACTN3抗体)，发现ACTN3缺失型(XX型)个体的肌肉代谢更依赖氧化途径，抗体验证了α-actinin-3表达缺失对能量代谢通路的影响。
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**备注**：上述文献为示例，实际引用时需核对真实来源及准确性。如需具体文献，建议通过PubMed或Web of Science以“ACTN3 antibody”或“α-actinin-3 detection”为关键词检索。
The ACTN3 gene encodes alpha-actinin-3. a sarcomeric protein predominantly expressed in fast-twitch skeletal muscle fibers, where it stabilizes the contractile apparatus and regulates energy metabolism. A common polymorphism (R577X) results in a premature stop codon, causing ACTN3 deficiency in approximately 20% of the global population. This genetic variation has been linked to athletic performance, with the functional allele (577R) associated with power/sprint-oriented traits, while the null variant (577X) correlates with endurance adaptations.
ACTN3 antibodies are essential tools for studying protein expression, localization, and function in muscle biology. They enable researchers to investigate ACTN3's role in muscle physiology, its interaction with dystrophin-associated proteins, and its implications in conditions like muscular dystrophy, sarcopenia, and metabolic disorders. Commercial ACTN3 antibodies are typically developed using immunogenic peptide sequences unique to the ACTN3 isoform, ensuring specificity over the homologous ACTN2 protein.
Applications include Western blotting, immunohistochemistry, and immunofluorescence to assess ACTN3 expression levels in tissue samples, cell cultures, or genetically modified models. Recent studies also utilize these antibodies to explore ACTN3's non-muscular roles, such as in cancer metastasis or cardiac remodeling. Despite advancements, challenges persist in antibody validation due to cross-reactivity risks and low ACTN3 expression in XX-genotype individuals, underscoring the need for rigorous experimental controls.
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