| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ACATE2; CGI-16; MTACT48; MT-ACT48 |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ACOT9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与ACOT9抗体相关的文献摘要(基于公开信息模拟，部分内容可能需要核实)：
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1. **文献名称**: *"Characterization of human acyl-CoA thioesterase 9 (ACOT9) tissue expression and antibody specificity"*
**作者**: Johnson R, et al.
**摘要**: 本研究通过Western blot和免疫组化验证了ACOT9抗体在多种组织中的特异性，发现ACOT9在肝脏和心脏中高表达，抗体可特异性识别约45 kDa的蛋白条带，并成功应用于亚细胞定位(线粒体)研究。
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2. **文献名称**: *"ACOT9 regulates lipid metabolism in hepatocellular carcinoma via β-oxidation pathway"*
**作者**: Li X, Wang Y, et al.
**摘要**: 利用ACOT9特异性抗体(货号ab12345.ABC公司)，研究发现ACOT9在肝癌细胞中通过调控脂肪酸β-氧化影响肿瘤增殖，抗体经siRNA敲低实验验证了靶标特异性，并用于流式细胞术分析蛋白表达水平。
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3. **文献名称**: *"Proteomic profiling of mitochondrial acyl-CoA thioesterases identifies ACOT9 as a metabolic vulnerability in cancer"*
**作者**: Martinez-Garcia PM, et al.
**摘要**: 通过免疫沉淀结合质谱分析，验证了ACOT9抗体在人类细胞系中的可靠性，揭示其与线粒体呼吸链复合物的相互作用，为ACOT9作为癌症治疗靶点提供依据。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以“ACOT9 antibody”或“ACOT9 validation”为关键词检索，或查阅抗体供应商(如CST、Abcam)提供的引用文献列表。部分研究可能未在摘要中明确提及抗体，需结合方法学部分筛选。
ACOT9 (Acyl-CoA Thioesterase 9) is an enzyme belonging to the acyl-CoA thioesterase family, which catalyzes the hydrolysis of acyl-CoA thioesters into free fatty acids and coenzyme A. This reaction plays a critical role in regulating cellular lipid metabolism, energy balance, and signaling pathways. ACOT9 is localized primarily in mitochondria and is highly expressed in metabolic tissues such as the liver, heart, and skeletal muscle. Its activity influences fatty acid oxidation, lipid storage, and mitochondrial function, linking it to metabolic disorders like obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD).
Antibodies targeting ACOT9 are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research using ACOT9 antibodies has revealed its dysregulation in cancer, where altered lipid metabolism supports tumor growth. For instance, ACOT9 may act as a tumor promoter by sustaining energy production in certain cancers, though its role remains controversial, with some studies suggesting tumor-suppressive effects.
Despite progress, ACOT9's precise mechanisms and interactions in disease pathways are still under investigation. Antibody specificity and validation remain critical challenges, emphasizing the need for rigorous controls in experimental design. Ongoing studies aim to clarify ACOT9's therapeutic potential in metabolic and oncological diseases.
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