| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AF9; YEATS3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MLLT3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MLLT3抗体相关的文献摘要信息：
1. **文献名称**: "The MLLT3/AF9 Gene is Expressed in Hematopoietic Progenitors and Committed Cells"
**作者**: Zeleznik-Le NJ et al.
**摘要**: 研究使用MLLT3抗体检测其在白血病细胞系和正常造血细胞中的表达，发现其在髓系分化中起调控作用，并与MLL基因易位相关。
2. **文献名称**: "AF9 (MLLT3) antibody characterization in chromatin remodeling studies"
**作者**: Wang Z et al.
**摘要**: 开发了一种特异性抗MLLT3单克隆抗体，验证其在染色质免疫共沉淀(ChIP)中的应用，证明MLLT3与组蛋白修饰复合物相互作用。
3. **文献名称**: "Role of MLLT3 in maintaining pluripotency of human embryonic stem cells"
**作者**: Chen J et al.
**摘要**: 通过MLLT3抗体进行蛋白定位分析，发现其通过调控Wnt/β-catenin通路维持干细胞多能性，敲低后导致分化异常。
4. **文献名称**: "Proteomic analysis identifies MLLT3 as a critical regulator in acute myeloid leukemia"
**作者**: Li Y et al.
**摘要**: 利用MLLT3抗体进行蛋白质组学筛选，揭示其在AML中通过调控HOX基因表达促进白血病细胞增殖的机制。
注：以上内容为示例性概括，实际文献需通过PubMed/Google Scholar以关键词“MLLT3 antibody”或“AF9 antibody”检索获取。
The MLLT3 antibody targets the MLLT3 protein, a key player in transcriptional regulation and chromatin remodeling. Also known as AF9. MLLT3 is encoded by the *MLLT3* gene on human chromosome 9p22 and belongs to the MLLT family of genes involved in chromosomal translocations. Notably, MLLT3 frequently partners with the *MLL* (KMT2A) gene in t(9;11)(p22;q23) translocations, driving aggressive subtypes of acute leukemia, particularly in infants. This fusion generates the MLL-AF9 oncoprotein, which disrupts epigenetic regulation by recruiting DOT1L, a histone methyltransferase, to aberrantly activate HOX gene clusters and sustain leukemic stem cells.
MLLT3 contains conserved domains, including a YEATS domain that binds acetylated histones, facilitating transcriptional elongation. Beyond leukemia, MLLT3 contributes to normal hematopoiesis and stem cell maintenance. Studies highlight its role in embryonic development, neural differentiation, and cancer metastasis. The MLLT3 antibody is widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). It aids in investigating MLL-rearranged leukemias, epigenetic dysregulation, and mechanisms of therapy resistance. Validated for specificity in human and murine models, this antibody remains essential for unraveling MLLT3's dual roles in oncogenesis and cellular homeostasis.
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