关键信息
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基因名
PPM1H
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简介
PPM1H, the enzyme subject, dephosphorylates CDKN1B at 'Thr-187,' critically regulating cellular processes. By preventing proteasomal degradation, PPM1H stabilizes CDKN1B, emphasizing its role in protein stabilization. This dephosphorylation event, orchestrated by PPM1H, intricately controls CDKN1B levels, showcasing its involvement in modulating cell cycle progression and regulating key cellular functions. PPM1H, Human (HEK293, His) is the recombinant human-derived PPM1H, expressed by HEK293 , with C-His labeled tag.
- 应用
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别名
Protein phosphatase 1H, PPM1H
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9ULR3
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表达区间
M1-S514
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氨基酸序列
MLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS
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蛋白长度
Full Length
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分子量
75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PPM1H, also known as Protein Phosphatase 2C Homolog H, is a member of the protein phosphatase 2C family, which plays a crucial role in regulating cellular signaling pathways by dephosphorylating serine and threonine residues on target proteins. This regulatory function is vital in numerous biological processes, including stress response, cell cycle regulation, and apoptosis. Recent studies have highlighted PPM1H’s involvement in various diseases, particularly cancer, where its dysregulation may contribute to tumorigenesis and the progression of malignant phenotypes. As an emerging therapeutic target, understanding the structure and function of PPM1H is essential, as it can provide insights into novel strategies for intervention in diseases characterized by aberrant signaling pathways. Recombinant PPM1H protein production allows for detailed biochemical and biophysical characterization, aiding in the study of its enzymatic activity, substrate specificity, and potential interactions with other cellular proteins. Additionally, the development of specific inhibitors or modulators of PPM1H may offer new avenues for therapeutic applications, particularly in the context of cancer treatment, where targeting phosphatases can be a promising strategy to combat tumor growth and resistance to existing therapies. Overall, the research on recombinant PPM1H protein is pivotal for elucidating its biological roles and therapeutic potential in various pathological conditions.
