| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AKAP15; AKAP18 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human AKAP7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AKAP7抗体的模拟参考文献示例(基于典型研究主题，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Subcellular localization of AKAP7γ in cardiac myocytes using a novel polyclonal antibody"
**作者**: Jones AB, et al.
**摘要**: 本研究开发了一种特异性识别AKAP7γ亚型的多克隆抗体，并通过Western blot和免疫荧光技术证实其在心肌细胞中的定位，发现AKAP7γ主要富集于细胞核周边区域，提示其参与调控心脏PKA信号通路。
2. **文献名称**: "AKAP7β interacts with PDE4D3 in spermatozoa: evidence from co-immunoprecipitation assays"
**作者**: Smith CD, et al.
**摘要**: 通过AKAP7β特异性抗体进行免疫共沉淀实验，揭示了AKAP7β与磷酸二酯酶PDE4D3在精子中的相互作用，表明其在精子活力调控中可能通过协调cAMP信号发挥作用。
3. **文献名称**: "Developmental expression of AKAP7 in murine testis: implications for spermatogenesis"
**作者**: Brown EF, et al.
**摘要**: 利用AKAP7抗体进行免疫组化分析，发现AKAP7在小鼠睾丸生精细胞中呈现阶段特异性表达，提示其参与精子发生过程中的微环境信号整合。
4. **文献名称**: "AKAP7 antibody validation for flow cytometry in human cardiomyocyte hypertrophy models"
**作者**: Li X, et al.
**摘要**: 验证了AKAP7抗体在人心肌细胞肥大模型中的流式细胞术适用性，发现AKAP7表达水平与病理肥厚标志物呈正相关，为疾病机制研究提供工具支持。
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**建议**：以上为模拟示例，实际文献请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词(如“AKAP7 antibody”、“AKAP7 immunolocalization”)，并筛选近年高影响力期刊的研究。
The AKAP7 antibody is a crucial tool for studying the A-kinase anchoring protein 7 (AKAP7), a member of the AKAP family that compartmentalizes protein kinase A (PKA) and other signaling molecules to specific subcellular locations. AKAP7 exists in multiple splice variants (e.g., γ, β, α) with distinct tissue expression and localization patterns, such as the plasma membrane, nucleus, or cytoplasm. It plays roles in regulating cardiac contractility, hormone secretion, and cell cycle progression by anchoring PKA to coordinate cAMP-responsive signaling pathways.
AKAP7 antibodies are designed to detect specific isoforms or shared epitopes, enabling applications like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help researchers investigate AKAP7's expression levels, cellular distribution, and interactions with binding partners (e.g., PKA, phosphodiesterases) under physiological or pathological conditions. Studies using AKAP7 antibodies have linked its dysregulation to cardiovascular diseases, reproductive disorders, and cancer progression.
Validated for specificity and sensitivity, AKAP7 antibodies (often raised in rabbits or mice) are essential for elucidating cAMP-PKA signaling mechanisms and exploring therapeutic targets. Their utility spans basic research and potential clinical diagnostics, particularly in diseases involving disrupted compartmentalized signaling.
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