| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KAIA0053 |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ARHGAP25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ARHGAP25抗体的3篇假设性参考文献示例(请注意文献为虚构，仅供参考格式)：
1. **文献名称**：*ARHGAP25 regulates neutrophil migration and immune response in sepsis*
**作者**：Li, X., Chen, Y., & Wang, H. (2022)
**摘要**：本研究利用ARHGAP25特异性抗体，揭示了该蛋白通过抑制RhoA信号通路负向调控中性粒细胞的迁移能力，在脓毒症模型中敲除ARHGAP25可加剧炎症反应，提示其作为潜在治疗靶点。
2. **文献名称**：*ARHGAP25 expression correlates with leukemia progression and drug resistance*
**作者**：Zhang, R., Liu, T., & Müller-Tidow, C. (2020)
**摘要**：通过免疫组化(ARHGAP25抗体验证)分析急性髓系白血病患者样本，发现ARHGAP25低表达与疾病进展和化疗耐药相关，机制涉及其对细胞骨架重塑的调控。
3. **文献名称**：*Structural insights into ARHGAP25-RhoGTPase interaction using monoclonal antibody targeting*
**作者**：Kuroda, S., Fukata, M., & Kaibuchi, K. (2019)
**摘要**：开发了一种高特异性ARHGAP25单克隆抗体，结合冷冻电镜技术解析了ARHGAP25与Rho GTP酶的结合界面，为设计小分子抑制剂提供了结构基础。
(注：以上内容为模拟生成，实际文献需通过PubMed等数据库检索确认。)
The ARHGAP25 antibody is a tool used to detect and study the ARHGAP25 protein, a member of the Rho GTPase-activating protein (RhoGAP) family. ARHGAP25. also known as RhoGAP interacting with CIP4 homolog 2 (RICH2), primarily regulates Rho GTPases, particularly Rac1 and Cdc42. by accelerating their GTP hydrolysis. This activity modulates cytoskeletal dynamics, cell migration, and adhesion, which are critical in immune responses and cancer progression.
ARHGAP25 is highly expressed in immune cells, such as neutrophils, where it influences phagocytosis, chemotaxis, and inflammatory signaling. Studies link its dysregulation to immune disorders, infections, and malignancies. For example, reduced ARHGAP25 expression in acute myeloid leukemia correlates with enhanced cell invasion, while its role in neutrophil function highlights its importance in innate immunity.
The ARHGAP25 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions in cellular and tissue samples. Researchers utilize it to explore ARHGAP25's mechanistic roles in diseases, including its tumor-suppressive effects in certain cancers or its regulatory functions in autoimmune conditions. Commercially available antibodies are typically validated for specificity, often targeting epitopes within the N-terminal or RhoGAP domains. Understanding ARHGAP25's biology through such antibodies provides insights into therapeutic strategies targeting Rho GTPase signaling pathways.
×
