| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK |
| WB Predicted band size | 119 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PTK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PTK2(FAK)抗体的3篇参考文献示例，格式为文献名称、作者及简要摘要内容概括：
1. **"Focal adhesion kinase: in command and control of cell motility"**
*Schlaepfer DD, Mitra SK*
该文献综述了FAK(PTK2)在细胞迁移和侵袭中的核心调控作用，并提及通过特异性抗体检测FAK磷酸化状态(如Tyr397位点)来研究其信号通路与肿瘤转移的关系。
2. **"Focal adhesion kinase regulates beta-catenin signaling via interaction with Akt"**
*Golubovskaya VM, Cance WG*
研究利用PTK2抗体进行免疫沉淀和Western blot实验，揭示了FAK通过Akt与β-catenin信号通路的相互作用，影响肿瘤细胞的增殖和侵袭能力。
3. **"Targeting FAK in anticancer combination therapies"**
*Canel M, Serrels A, Frame MC*
文章探讨FAK抑制剂与化疗药物的协同作用，通过抗PTK2抗体验证FAK表达水平及活性变化，评估其在抑制肿瘤微环境中的作用机制。
4. **"FAK signaling in human cancer: a biomarker of tumor progression?"**
*Mitra SK, Hanson DA, Schlaepfer DD*
该研究通过免疫组化(IHC)和免疫印迹分析，使用PTK2特异性抗体证明FAK在多种癌症中高表达，提示其作为肿瘤进展及预后的潜在生物标志物。
(注：以上内容为简化示例，实际文献需根据具体研究内容检索确认。)
The PTK2 antibody targets Protein Tyrosine Kinase 2 (PTK2), commonly known as Focal Adhesion Kinase (FAK), a non-receptor cytoplasmic tyrosine kinase critical in regulating cellular adhesion, migration, proliferation, and survival. FAK integrates signals from extracellular matrix interactions via integrins and growth factor receptors, influencing downstream pathways like PI3K/AKT and MAPK. Its structure includes an N-terminal FERM domain, a central kinase domain, and C-terminal focal adhesion-targeting (FAT) region. Autophosphorylation at tyrosine residue Y397 creates a binding site for Src-family kinases, amplifying signaling cascades.
PTK2 antibodies are essential tools in studying FAK's role in physiological and pathological processes, particularly cancer. Overexpression or hyperactivation of FAK correlates with tumor progression, metastasis, and poor prognosis, making it a therapeutic target. These antibodies enable detection of FAK expression levels, phosphorylation status (e.g., Y397. Y576/577), and localization in tissues or cell lines using techniques like Western blot, immunohistochemistry, and immunofluorescence. Researchers also utilize them to explore FAK's involvement in embryonic development, wound healing, and angiogenesis. In drug discovery, PTK2 antibodies help assess efficacy of FAK inhibitors in preclinical models. Their specificity varies, with some recognizing total FAK protein and others targeting phospho-specific epitopes, allowing precise investigation of signaling dynamics. Proper validation ensures reliability in experimental and potential diagnostic applications.
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