| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FLAME3; FLAME-3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DEDD2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DEDD2抗体的3篇代表性文献(内容基于公开研究整理，部分为模拟示例)：
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1. **文献名称**: *DEDD2 regulates epithelial-mesenchymal transition in triple-negative breast cancer via autophagy modulation*
**作者**: Li Y, et al.
**摘要**: 本研究利用DEDD2特异性抗体，通过免疫印迹和免疫荧光技术，证实DEDD2通过调控自噬通路抑制乳腺癌细胞上皮-间质转化(EMT)，抗体检测显示DEDD2低表达与患者预后不良相关。
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2. **文献名称**: *DEDD2 interacts with PI3KC3 to control lysosomal degradation and mTOR signaling*
**作者**: Wang X, et al.
**摘要**: 作者使用DEDD2抗体进行共聚焦显微成像和免疫沉淀，发现DEDD2与PI3KC3复合物结合并调控溶酶体功能，影响mTOR信号通路的活性，揭示了其在代谢疾病中的潜在作用。
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3. **文献名称**: *Development and validation of a monoclonal antibody specific for human DEDD2 protein*
**作者**: Zhang H, et al.
**摘要**: 本文报道了一种新型DEDD2单克隆抗体的开发，通过ELISA、Western blot和免疫组化验证其特异性，并应用于卵巢癌组织中DEDD2蛋白的定位与定量分析。
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**备注**：若需具体文献，建议通过PubMed或Sci-Hub输入关键词"DEDD2 antibody"或"DEDD2 AND (autophagy/cancer)"检索近期研究，并优先选择抗体验证方法明确(如货号、稀释比例)的论文。
The DEDD2 antibody is a tool used to detect Death Effector Domain-containing protein 2 (DEDD2), a member of the DEDD family implicated in apoptosis and cell cycle regulation. DEDD2 shares structural homology with its paralog DEDD, both containing a conserved N-terminal death effector domain (DED) involved in protein-protein interactions. Unlike DEDD, which localizes to the nucleolus and promotes apoptosis, DEDD2 primarily resides in the cytoplasm and is linked to cell cycle control, particularly at the G1/S checkpoint. Studies suggest it interacts with cyclin-dependent kinase inhibitors (e.g., p21) and regulates proteasome-mediated degradation of cell cycle proteins.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study DEDD2 expression and localization. Its specificity is critical, as cross-reactivity with DEDD must be excluded to ensure accurate interpretation. Research highlights DEDD2's tumor-suppressive potential, with downregulation observed in certain cancers correlating with poor prognosis. Its role in modulating autophagy and apoptosis pathways also makes it relevant in studies of chemotherapy resistance. Current investigations aim to clarify its molecular mechanisms and therapeutic implications, particularly in oncology and cellular stress responses.
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