| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CG; CATG |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CTSG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于FANCF抗体的3篇参考文献示例(注：内容为模拟概括，实际文献需根据具体研究检索)：
1. **文献名称**：*"FANCF forms a nuclear complex with other Fanconi anemia core complex proteins involved in DNA repair"*
**作者**：de Winter et al.
**摘要**：研究通过免疫共沉淀和蛋白质印迹技术，利用FANCF抗体验证了其与FANCA、FANCG等蛋白的相互作用，证实FANCF在FA复合体组装中的关键作用，并参与DNA交联损伤修复。
2. **文献名称**：*"Functional analysis of Fanconi anemia mutations in FANCF identifies critical residues for protein stability"*
**作者**：Timmers et al.
**摘要**：通过Western blot和免疫荧光技术，使用特异性FANCF抗体检测患者细胞中FANCF蛋白表达水平及亚细胞定位，揭示了特定基因突变导致蛋白稳定性下降的机制。
3. **文献名称**：*"FANCF silencing contributes to increased chemosensitivity in ovarian cancer cells"*
**作者**：Zhou et al.
**摘要**：研究通过siRNA敲低FANCF并结合抗体检测蛋白表达，发现FANCF缺失可增强卵巢癌细胞对顺铂的敏感性，提示其作为潜在治疗靶点的价值。
(建议通过PubMed或Google Scholar以“FANCF antibody”或“FANCF protein analysis”为关键词检索近期文献，获取具体实验方法和抗体应用细节。)
The FANCF antibody is a crucial tool for studying Fanconi anemia (FA), a rare genetic disorder characterized by bone marrow failure, congenital abnormalities, and cancer predisposition. FANCF is a core component of the FA/BRCA DNA repair pathway, which coordinates the repair of DNA interstrand crosslinks (ICLs) and maintains genomic stability. The FANCF protein interacts with other FA complex members (e.g., FANCA, FANCG) to facilitate monoubiquitination of FANCD2/FANCI, a critical step in recruiting downstream repair effectors. Mutations in FANCF are linked to FA complementation group F and are associated with early-onset cancers, such as leukemia and solid tumors.
FANCF antibodies are widely used in research to detect FANCF protein expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate FA pathway activation, DNA damage responses, and mechanisms of chemotherapeutic resistance. Commercially available antibodies are typically raised against specific epitopes of human FANCF, with validation in knockout cell lines to ensure specificity. Dysregulation of FANCF has also been observed in sporadic cancers, making its detection relevant for studying genomic instability in malignancies. Additionally, FANCF antibodies aid in diagnosing FA and understanding its molecular pathology, particularly in identifying functional defects in the FA/BRCA network. Their application extends to exploring synthetic lethal therapies targeting FA-deficient cancers, highlighting both clinical and research significance.
×
