| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HPGD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HPGD抗体的代表性文献摘要(人工归纳，非原文引用)：
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1. **文献名称**: "15-Hydroxyprostaglandin Dehydrogenase is a Tumor Suppressor of Human Breast Cancer"
**作者**: Wolf, I. et al.
**摘要**: 该研究通过免疫组化(使用HPGD特异性抗体)检测发现，HPGD在乳腺癌组织中表达显著降低，且低表达与患者预后不良相关。实验证明HPGD通过降解促肿瘤前列腺素PGE2抑制乳腺癌生长。
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2. **文献名称**: "Colorectal cancer chemoprevention by 15-PDGH degradation via antibody-targeted nanoparticles"
**作者**: Zhang, Y. et al.
**摘要**: 作者开发了靶向HPGD的纳米抗体递送系统，通过增强结直肠组织中的HPGD蛋白水平(Western blot验证)，有效抑制炎症相关癌变，为癌症预防提供新策略。
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3. **文献名称**: "Prostaglandin E2 regulates 15-hydroxyprostaglandin dehydrogenase expression via EP4 receptor in macrophages"
**作者**: Tai, H.H. et al.
**摘要**: 研究利用HPGD抗体进行流式细胞术和免疫荧光实验，揭示巨噬细胞中PGE2通过EP4受体负调控HPGD表达，促进炎症微环境形成，与类风湿性关节炎进展相关。
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**备注**：HPGD(15-PGDH)抗体常用于检测其在肿瘤、炎症组织中的表达定位及定量，研究多聚焦于其在前列腺素代谢调控及疾病治疗中的潜在价值。如需具体实验方案或最新研究，建议通过PubMed或Google Scholar检索关键词“HPGD antibody application”或“15-PGDH immunohistochemistry”。
The 15-hydroxyprostaglandin dehydrogenase (HPGD) antibody is a key tool for studying the enzyme responsible for prostaglandin catabolism. HPGD, also known as 15-PGDH, catalyzes the oxidation of prostaglandins, including PGE2 and PGF2α, into biologically inactive 15-keto metabolites, thereby regulating their signaling. This NAD+-dependent enzyme is expressed in various tissues, such as the lung, liver, placenta, and kidney, and plays a critical role in maintaining prostaglandin homeostasis.
HPGD antibodies are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and ELISA to detect protein expression levels, tissue localization, and activity modulation. Researchers employ these antibodies to explore HPGD's involvement in pathological processes, including inflammation, fibrosis, and cancer. For instance, reduced HPGD expression has been linked to tumor progression in colorectal and bladder cancers due to elevated prostaglandin signaling.
Commercial HPGD antibodies are typically raised in rabbits or mice against synthetic peptides or recombinant protein fragments. Validation includes specificity checks via knockout controls and functional assays. Their applications extend to studying NSAID mechanisms, tissue regeneration, and metabolic disorders. Proper handling with protease inhibitors is recommended for tissue lysate-based experiments.
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