| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HSD3B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与HSD3B1抗体相关的假设性参考文献示例(注：部分文献信息为模拟内容，实际引用请核实)：
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1. **《HSD3B1 Expression in Castration-Resistant Prostate Cancer》**
- 作者：Thomas, R. et al.
- 摘要：研究通过免疫组化(使用HSD3B1特异性抗体)发现，HSD3B1蛋白在前列腺癌去势抵抗性患者肿瘤组织中过表达，其高表达与类固醇合成通路上调及阿比特龙治疗耐药相关。
2. **《Tissue-Specific HSD3B1 Isoform Characterization Using Monoclonal Antibodies》**
- 作者：Chang, C.Y. & Lin, D.
- 摘要：开发针对HSD3B1不同异构体的单克隆抗体，通过Western blot和免疫荧光验证其在肾上腺、性腺及肿瘤组织中的特异性表达差异，揭示其在类固醇代谢中的组织特异性功能。
3. **《HSD3B1 Mutation-Driven Alterations in Androgen Biosynthesis》**
- 作者：Sharifi, N. & Agarwal, V.
- 摘要：研究HSD3B1基因突变(如p.1245A)对酶活性及蛋白稳定性的影响，利用抗体检测突变体蛋白表达水平，证实其导致细胞内DHT合成增强，促进前列腺癌进展。
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**备注**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台以关键词“HSD3B1 antibody”、“HSD3B1 immunohistochemistry”或“HSD3B1 protein expression”检索真实文献。
The HSD3B1 antibody is a crucial tool in studying the enzyme 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase type 1 (3β-HSD1), encoded by the HSD3B1 gene. This enzyme plays a pivotal role in steroidogenesis, catalyzing the conversion of Δ5 steroids (e.g., pregnenolone, 17α-hydroxypregnenolone, dehydroepiandrosterone) to Δ4 steroids (progesterone, 17α-hydroxyprogesterone, androstenedione), essential for synthesizing active steroid hormones like cortisol, aldosterone, and sex hormones. Dysregulation of HSD3B1 is linked to endocrine disorders (e.g., congenital adrenal hyperplasia) and hormone-dependent cancers (prostate, breast).
HSD3B1 antibodies are widely used in research to detect protein expression and localization in tissues, including adrenal glands, gonads, and peripheral steroidogenic sites. They enable investigations into enzyme function, hormonal regulation, and disease mechanisms. For example, in prostate cancer, HSD3B1 variants affecting enzyme stability influence androgen biosynthesis and treatment resistance. Antibodies are validated in techniques like Western blotting, immunohistochemistry, and immunofluorescence, often supported by knockout controls.
Recent studies also explore HSD3B1's role in metabolic syndromes and its potential as a therapeutic target. The antibody's specificity and reliability make it vital for both basic research and clinical applications, bridging molecular insights with diagnostic or therapeutic advancements.
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