| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KAR; SDR12C1 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HSD17B12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HSD17B12抗体的3篇参考文献示例(注：以下内容为示例性概括，具体文献需根据实际数据库检索确认)：
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1. **文献名称**: *HSD17B12 Expression in Breast Cancer: Correlation with Hormone Receptor Status and Prognosis*
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化(IHC)和Western blot分析乳腺癌组织中HSD17B12的表达，发现其高表达与雌激素受体阳性(ER+)亚型及患者不良预后显著相关，提示HSD17B12可能作为治疗靶点。
2. **文献名称**: *Role of HSD17B12 in Lipid Metabolism: Insights from Knockout Mouse Models*
**作者**: Zhang L, et al.
**摘要**: 利用HSD17B12特异性抗体检测基因敲除小鼠的肝脏组织，发现该酶缺失导致长链脂肪酸合成受阻，表明HSD17B12在脂类代谢中起关键作用。
3. **文献名称**: *HSD17B12 as a Potential Biomarker in Ovarian Cancer: Validation by Immunohistochemistry*
**作者**: Tanaka K, et al.
**摘要**: 通过组织芯片和HSD17B12抗体进行大规模IHC分析，证实其在卵巢癌中的高表达与肿瘤进展和化疗耐药性相关，支持其作为预后标志物的潜力。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索具体文献(关键词：HSD17B12 antibody, cancer, lipid metabolism)。如需真实文献，建议查阅近年相关领域论文或数据库。
The HSD17B12 antibody is a crucial tool for studying the 17β-hydroxysteroid dehydrogenase type 12 (HSD17B12) enzyme, a member of the HSD17B family involved in steroid metabolism and lipid biosynthesis. HSD17B12 catalyzes the reduction of 17-ketosteroids to 17β-hydroxysteroids and plays a key role in elongating very long-chain fatty acids (VLCFAs) within the endoplasmic reticulum. This enzyme is critical for cellular membrane integrity, signaling, and lipid homeostasis. Dysregulation of HSD17B12 has been implicated in cancers, including breast, prostate, and liver malignancies, where its overexpression correlates with tumor progression, metastasis, and therapy resistance.
HSD17B12 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and quantification in tissues or cell lines. They aid in exploring HSD17B12's functional roles, such as its interaction with lipid metabolism pathways or hormone-dependent signaling. Commercially available antibodies are typically validated for specificity, often targeting epitopes within conserved regions of the protein. Recent studies highlight its potential as a diagnostic biomarker or therapeutic target, particularly in cancers with altered lipid metabolism. However, challenges remain in standardizing antibody performance across experimental conditions, necessitating rigorous validation to ensure reproducibility in research applications.
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