| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MDH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MDH1抗体的3篇示例参考文献(内容为虚构示例，供格式参考)：
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1. **文献名称**: *MDH1 overexpression in colorectal cancer: Prognostic implications and metabolic reprogramming*
**作者**: Smith A, et al.
**摘要**: 本研究利用MDH1特异性抗体通过免疫组化(IHC)分析结直肠癌组织，发现MDH1蛋白表达显著升高，且与患者不良预后相关。进一步实验表明，MDH1通过调节NAD+/NADH平衡促进肿瘤细胞代谢重编程。
2. **文献名称**: *Validation of a novel MDH1 monoclonal antibody for Western blot and immunofluorescence applications*
**作者**: Chen L, et al.
**摘要**: 文章报道了一种高特异性MDH1单克隆抗体的开发与验证。该抗体在Western blot中成功检测到MDH1的敲低和过表达，并在免疫荧光实验中定位MDH1于线粒体，为代谢疾病研究提供了可靠工具。
3. **文献名称**: *MDH1 as a potential biomarker in hepatocellular carcinoma: Insights from antibody-based detection*
**作者**: Wang X, et al.
**摘要**: 通过MDH1抗体对肝癌组织进行染色，研究发现MDH1低表达与肿瘤侵袭性相关。机制研究表明，MDH1缺失导致线粒体功能障碍，可能通过活性氧(ROS)积累促进肝癌进展。
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**说明**：以上文献为示例，实际引用时需检索PubMed、Web of Science等数据库获取真实研究。如需进一步协助查找，请提供更多信息。
Malate dehydrogenase 1 (MDH1) is a mitochondrial enzyme that catalyzes the reversible oxidation of malate to oxaloacetate, playing a critical role in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. This enzyme is essential for cellular energy production, amino acid synthesis, and redox balance. MDH1 antibodies are immunological tools designed to detect and quantify MDH1 protein expression in various biological samples. They are widely used in research to study mitochondrial metabolism, cellular responses to metabolic stress, and diseases linked to mitochondrial dysfunction, such as cancer, neurodegenerative disorders, and metabolic syndromes.
MDH1 antibodies are commonly applied in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein localization, expression levels, and post-translational modifications. Recent studies highlight MDH1's potential role in cancer progression, where its dysregulation may influence tumor growth, metastasis, or therapeutic resistance. In neurodegenerative contexts, altered MDH1 activity has been associated with mitochondrial impairment in conditions like Alzheimer’s disease.
The development and validation of MDH1 antibodies require careful consideration of cross-reactivity with homologous proteins (e.g., MDH2) to ensure specificity. These antibodies serve as vital reagents for unraveling MDH1's functional diversity, offering insights into metabolic adaptations in health and disease.
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