| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | L39mt; MRPL5; RPML5; MRP-L5; PRED22; PRED66; MSTP003; C21orf92 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL39 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL39抗体的模拟参考文献示例(注：以下文献为虚构示例，实际文献请通过学术数据库检索确认)：
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1. **文献名称**：*MRPL39 is essential for mitochondrial ribosome biogenesis and cellular respiration*
**作者**：Chen L, et al.
**摘要**：本研究利用CRISPR-Cas9技术敲除MRPL39基因，发现其缺失导致线粒体核糖体大亚基组装缺陷。通过特异性MRPL39抗体进行Western blot和免疫荧光实验，证实MRPL39蛋白在线粒体内的定位，并揭示其功能缺失引发细胞能量代谢障碍。
2. **文献名称**：*A novel MRPL39 antibody reveals tissue-specific expression patterns in mammalian mitochondria*
**作者**：Kim S, et al.
**摘要**：作者开发了一种高特异性MRPL39多克隆抗体，并通过免疫组化、免疫印迹验证其在心脏、肝脏等高代谢组织中的高表达。该抗体为研究MRPL39在疾病中的表达变化提供了可靠工具。
3. **文献名称**：*MRPL39 mutations impair mitochondrial translation and cause neurodevelopmental disorders*
**作者**：Wang X, et al.
**摘要**：通过全基因组测序发现MRPL39基因突变与儿童神经发育异常相关。研究使用MRPL39抗体检测患者细胞系，发现突变导致蛋白表达量显著降低，并伴随线粒体呼吸链复合物合成受损。
4. **文献名称**：*MRPL39 regulates cancer cell proliferation via modulating mitochondrial protein synthesis*
**作者**：Garcia R, et al.
**摘要**：在乳腺癌模型中，MRPL39抗体用于评估其表达水平与肿瘤生长的关系。结果显示，MRPL39敲低通过抑制线粒体翻译活性，显著减缓癌细胞增殖。
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**建议**：如需真实文献，请通过PubMed、Google Scholar等平台检索关键词“MRPL39 antibody”、“MRPL39 mitochondrial ribosome”或结合具体研究领域(如癌症、线粒体疾病)进一步筛选。
The MRPL39 antibody is a research tool designed to detect the mitochondrial ribosomal protein L39 (MRPL39), a component of the large subunit (39S) of the mitochondrial ribosome. MRPL39 plays a critical role in mitochondrial protein synthesis, supporting the assembly of oxidative phosphorylation (OXPHOS) complexes essential for ATP production. As mitochondria are central to cellular energy metabolism and apoptosis, MRPL39 is implicated in maintaining mitochondrial function and cellular homeostasis. Dysregulation of mitochondrial ribosomes, including MRPL39. has been linked to metabolic disorders, neurodegenerative diseases, and cancer.
The MRPL39 antibody is widely used in studies investigating mitochondrial dysfunction, particularly in contexts like tumorigenesis, where altered mitochondrial metabolism is a hallmark. Researchers employ this antibody in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess MRPL39 expression levels, localization, and interactions. Validation often includes testing in knockdown models or mitochondrial-enriched fractions to confirm specificity.
Commercial MRPL39 antibodies are typically raised in hosts like rabbits or mice, with clones validated for cross-reactivity in human, mouse, or rat samples. Proper controls, such as comparing wild-type and mitochondrial-depleted cells, are recommended to ensure accurate interpretation. Understanding MRPL39's role through antibody-based assays contributes to insights into mitochondrial diseases and therapeutic strategies targeting cellular energetics.
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