| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | B13; NUFM; UQOR13; CI-13kB; CI-13KD-B |
| WB Predicted band size | 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NDUFA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFA5抗体的3篇示例参考文献(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "NDUFA5 expression is reduced in Parkinson's disease cybrid cell models"
**作者**: Smith J, et al.
**摘要**: 本研究利用NDUFA5抗体通过Western blot分析帕金森病细胞模型中线粒体复合体I亚基的表达水平，发现NDUFA5蛋白显著下调，提示其可能参与多巴胺能神经元的线粒体功能障碍。
2. **文献名称**: "Antibody-based profiling of mitochondrial complex I subunits in colorectal cancer"
**作者**: Lee S, et al.
**摘要**: 通过免疫组织化学(IHC)和免疫印迹(WB)技术，使用NDUFA5抗体检测结直肠癌组织中线粒体复合体I的分布及表达量，发现NDUFA5的低表达与患者预后不良相关。
3. **文献名称**: "Characterization of a novel NDUFA5 knockout mouse using specific polyclonal antibodies"
**作者**: García-Ruiz C, et al.
**摘要**: 研究团队开发并验证了一种高特异性NDUFA5多克隆抗体，用于检测基因敲除小鼠模型中NDUFA5蛋白的缺失，证实其在维持复合体I组装中的关键作用。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“NDUFA5 antibody”、“NDUFA5 mitochondrial complex I”等，并筛选应用该抗体的实验研究。
The NDUFA5 antibody is a crucial tool for studying mitochondrial complex I (NADH:ubiquinone oxidoreductase), a key component of the electron transport chain. NDUFA5 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5) is a nuclear-encoded subunit of complex I, which plays a vital role in ATP synthesis by facilitating electron transfer from NADH to ubiquinone. Dysregulation of NDUFA5 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and certain cancers.
NDUFA5 antibodies are widely used in research to detect protein expression levels via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate mitochondrial dysfunction mechanisms, assess complex I assembly defects, and explore metabolic adaptations in diseases. Species specificity typically covers human, mouse, and rat samples, with common hosts including rabbit (polyclonal) and mouse (monoclonal).
Validation of NDUFA5 antibodies often involves knockout cell lines or tissue controls to confirm specificity. Recent studies also utilize these antibodies to probe NDUFA5’s role in oxidative stress responses and its potential as a biomarker for metabolic syndromes. Researchers must optimize experimental conditions, as mitochondrial protein extraction and antibody dilution ratios can significantly impact results.
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