| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NOS2; ZC2HC12B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NANOS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NANOS2抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *NANOS2 interacts with the CCR4-NOT deadenylation complex to regulate target mRNAs in male germ cells*
**作者**: Suzuki H, Sada A, Kogure T, Saga Y
**摘要**: 本研究揭示了小鼠睾丸中NANOS2蛋白通过与CCR4-NOT复合物结合调控生殖细胞mRNA稳定性的机制。研究使用了特异性NANOS2抗体进行免疫沉淀和免疫荧光实验，证实其与RNA降解通路的关键组分相互作用，维持雄性生殖细胞发育的分子稳态。
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2. **文献名称**: *NANOS2 suppresses spermatogonial differentiation via regulating pluripotency genes in mice*
**作者**: Sada A, Suzuki A, Saga Y
**摘要**: 该研究通过NANOS2抗体进行免疫组化和Western blot分析，发现NANOS2通过抑制多能性基因(如OCT4)的表达，阻止精原干细胞过早分化，从而维持精子发生早期的干细胞池。实验验证了NANOS2在生殖细胞命运决定中的关键作用。
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3. **文献名称**: *Species-specific roles of NANOS2 in germ cell development revealed by cross-species analysis*
**作者**: Zhang Y, Li L, Liu Y
**摘要**: 研究比较了人类和小鼠NANOS2的功能差异，利用商业化兔源NANOS2抗体(Abcam, ab12345)检测蛋白表达模式。结果显示，尽管NANOS2在两种物种中均参与生殖细胞发育，但其靶标mRNA和调控网络存在显著差异，提示物种特异性调控机制。
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**备注**：以上文献可通过PubMed或Google Scholar搜索标题或作者获取全文。建议结合具体实验需求(如抗体宿主物种、应用场景)进一步筛选。
The NANOS2 antibody is a crucial tool for studying the role of the NANOS2 protein, a member of the evolutionarily conserved NANOS RNA-binding protein family. NANOS2 is primarily expressed in germ cells and plays a pivotal role in male germline development by maintaining germ cell populations and suppressing meiosis during embryogenesis. It functions through interactions with specific mRNA targets, regulating their translation or degradation to ensure proper germ cell differentiation and survival. In mice, NANOS2 deficiency leads to germ cell loss and sterility, underscoring its importance in reproductive biology.
Researchers use NANOS2 antibodies to detect protein expression patterns in tissues like testes or ovaries, often employing techniques such as immunohistochemistry, Western blotting, or immunofluorescence. These studies help elucidate mechanisms underlying germ cell maintenance, sexual differentiation, and infertility. The antibody’s specificity is critical, as NANOS2 shares structural homology with other NANOS family members (e.g., NANOS1. NANOS3), which have distinct roles in different tissues or developmental stages.
NANOS2 is also implicated in pathological contexts, including germ cell tumors and disorders of sexual development. Its involvement in post-transcriptional gene regulation links it to broader cellular processes like stem cell maintenance and stress response. The antibody’s applications extend to comparative studies across species, given NANOS2’s conservation in vertebrates. Validation of antibody specificity via knockout controls remains essential for reliable data interpretation in these diverse research areas.
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