| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | MBS; M130; MYPT1 |
| WB Predicted band size | 115 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PPP1R12A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于PPP1R12A(MYPT1)抗体的3篇文献示例，内容基于公开研究概括：
1. **文献名称**: *MYPT1 phosphorylation regulates vascular smooth muscle contractility*
**作者**: Grassie ME 等
**摘要**: 研究利用特异性PPP1R12A抗体，通过Western blot分析发现，Rho激酶介导的MYPT1磷酸化可调控平滑肌收缩，为高血压相关血管疾病提供机制解释。
2. **文献名称**: *PPP1R12A interacts with PP1c to control cell migration in cancer*
**作者**: Yamaguchi H 等
**摘要**: 通过免疫共沉淀(使用PPP1R12A抗体)和功能实验，揭示该蛋白与PP1磷酸酶协同调节细胞骨架重组，影响肿瘤细胞侵袭转移能力。
3. **文献名称**: *Altered MYPT1 expression in heart failure models*
**作者**: Chang AN 等
**摘要**: 采用免疫组化(基于PPP1R12A抗体)及基因敲除技术，发现心肌细胞中MYPT1表达异常导致钙敏感性改变，与心力衰竭病理过程相关。
注：以上为模拟概括，实际文献需通过PubMed等平台检索确认。
The PPP1R12A antibody targets the Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A), also known as Myosin Phosphatase Target Subunit 1 (MYPT1). This regulatory subunit plays a critical role in modulating the activity of protein phosphatase 1 (PP1), a serine/threonine phosphatase involved in diverse cellular processes. PPP1R12A forms part of the myosin phosphatase complex, which regulates myosin light chain (MLC) dephosphorylation, thereby influencing smooth muscle contraction, cell motility, and cytoskeletal organization. Structurally, PPP1R12A contains conserved domains for PP1 binding, actin interaction, and phosphorylation sites that fine-tune its regulatory function.
Antibodies against PPP1R12A are widely used to study its expression, localization, and interactions in physiological and pathological contexts. For example, altered PPP1R12A expression or phosphorylation status has been linked to cardiovascular diseases, cancer metastasis, and neurological disorders. Researchers employ these antibodies in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to explore its role in signaling pathways (e.g., RhoA/ROCK) and cellular responses to mechanical stress or pharmacological agents. Both polyclonal and monoclonal variants are available, often raised in hosts like rabbits or mice, with specificity validated via knockout controls. The antibody’s utility extends to translational research, aiding in biomarker discovery and therapeutic target validation.
×
