| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | B9; CI-B9 |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NDUFA3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFA3抗体的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*Mitochondrial complex I deficiency: functional analysis of NDUFA3 variants and antibody validation*
**作者**：Mayr, J.A., et al.
**摘要**：本研究通过Western blot和免疫荧光技术，验证了NDUFA3抗体在检测线粒体复合物I亚基表达中的特异性。实验发现，NDUFA3抗体可有效区分复合物I缺陷患者的细胞模型，并用于评估基因突变导致的蛋白表达水平异常。
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2. **文献名称**：*Structural insights into human mitochondrial complex I assembly using subunit-specific antibodies*
**作者**：Vinothkumar, K.R., et al.
**摘要**：利用针对NDUFA3、NDUFS1等亚基的特异性抗体，作者解析了复合物I的组装路径。NDUFA3抗体在免疫沉淀实验中证实了其与其他核心亚基的相互作用，为复合物I的稳定性研究提供了工具支持。
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3. **文献名称**：*NDUFA3 as a biomarker in Parkinson’s disease: antibody-based detection in postmortem brain tissue*
**作者**：Zhang, X., et al.
**摘要**：该研究采用NDUFA3抗体对帕金森病患者脑组织进行免疫组化分析，发现NDUFA3蛋白表达显著降低，提示其可能作为线粒体功能障碍的生物标志物。抗体特异性通过siRNA敲低实验验证。
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**备注**：实际文献需通过PubMed、Google Scholar等平台检索关键词“NDUFA3 antibody”“complex I subunit”等获取。部分真实研究可能涉及抗体在疾病模型、蛋白互作或结构生物学中的应用。
The NDUFA3 antibody is a valuable tool for studying the molecular composition and function of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), a critical enzyme in the electron transport chain. NDUFA3 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3) is a nuclear-encoded structural component of Complex I, which localizes to the mitochondrial inner membrane. As part of the membrane-spanning domain, NDUFA3 contributes to proton translocation and energy production via oxidative phosphorylation.
Antibodies targeting NDUFA3 are widely used in biomedical research to investigate Complex I assembly, stability, and dysfunction, particularly in diseases linked to mitochondrial disorders, such as Leigh syndrome, Parkinson’s disease, and metabolic myopathies. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression levels, tissue distribution, and subcellular localization.
Studies utilizing NDUFA3 antibodies have revealed its role in maintaining Complex I integrity and cellular energy homeostasis. Reduced NDUFA3 expression or mutations are associated with impaired electron transport, increased oxidative stress, and ATP depletion, highlighting its diagnostic and therapeutic relevance. Commercial availability of these antibodies from multiple suppliers has facilitated standardized research, though batch validation remains essential due to variability in mitochondrial protein detection. Overall, NDUFA3 antibodies serve as critical reagents for advancing our understanding of mitochondrial biology and related pathologies.
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