| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RSP5; NEDD4-2; NEDD4.2; hNEDD4-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NEDD4L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEDD4L抗体的3篇代表性文献示例(注：文献信息为模拟，建议通过学术数据库核实)：
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1. **文献名称**: *"NEDD4L regulates the ubiquitination and stability of the epithelial sodium channel (ENaC)"*
**作者**: Staub O, Rotin D
**摘要**: 本研究利用特异性NEDD4L抗体，通过免疫共沉淀和Western blot技术，揭示了NEDD4L通过泛素化修饰调控ENaC通道蛋白的稳定性，影响肾脏钠离子重吸收的分子机制。
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2. **文献名称**: *"NEDD4L suppresses tumor growth by targeting EGFR for lysosomal degradation"*
**作者**: Wang X, Levchenko A
**摘要**: 通过开发高特异性NEDD4L单克隆抗体，作者发现NEDD4L在肺癌中低表达，并证实其通过促进EGFR降解抑制肿瘤增殖，为癌症治疗提供潜在靶点。
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3. **文献名称**: *"A validated NEDD4L antibody toolkit for studying neural development"*
**作者**: Kumar S, et al.
**摘要**: 本文报道了一种经质谱验证的兔源NEDD4L多克隆抗体的开发，并应用于免疫组化和流式细胞术，证明NEDD4L在小鼠脑发育过程中动态表达，参与神经元分化调控。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“NEDD4L antibody”或结合研究领域(如癌症、肾脏病等)筛选近期论文。
NEDD4L (Neural precursor cell Expressed Developmentally Down-regulated 4-Like) is a HECT-type E3 ubiquitin ligase involved in regulating protein turnover via the ubiquitin-proteasome system. It plays critical roles in cellular processes such as ion channel regulation, membrane protein trafficking, and signaling pathway modulation. Structurally, NEDD4L contains a C2 domain (mediating membrane binding), multiple WW domains (substrate recognition), and a catalytic HECT domain. Its activity is tightly controlled through phosphorylation (e.g., by SGK1) and alternative splicing, particularly in regulatory regions affecting substrate specificity.
NEDD4L antibodies are essential tools for studying its expression patterns, subcellular localization, and interactions with substrates like ENaC (epithelial sodium channel) and SMAD2/3. These antibodies enable detection through techniques including Western blotting, immunohistochemistry, and immunoprecipitation. Research applications span hypertension studies (via ENaC regulation in kidneys), cancer biology (modulating PTEN/TP53 pathways), and neurological disorders (synaptic protein regulation). Validated antibodies help distinguish between NEDD4L isoforms generated by alternative promoters and splicing events. As NEDD4L dysfunction is implicated in Liddle syndrome, pulmonary hypertension, and tumor progression, specific antibodies support both mechanistic studies and therapeutic development targeting its ubiquitination activity. Proper antibody validation via knockout controls remains crucial given sequence similarities within the NEDD4 ubiquitin ligase family.
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