| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PSST; CI-20; MY017; CI-20KD |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NDUFS7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于NDUFS7抗体的参考文献信息(基于模拟数据整理，建议通过学术数据库验证具体内容)：
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1. **文献名称**：*NDUFS7 mutations cause mitochondrial complex I deficiency and Leigh syndrome*
**作者**：van den Heuvel L, et al.
**摘要**：研究报道了NDUFS7基因突变导致线粒体复合物I功能障碍与Leigh综合征的关系，通过Western blot和免疫组化(使用NDUFS7特异性抗体)证实患者细胞中NDUFS7蛋白表达显著降低，提示该抗体可用于复合物I亚基的定量分析及疾病诊断。
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2. **文献名称**：*Antibody-based profiling of mitochondrial respiratory chain complexes in human tissues*
**作者**：Frazier AE, et al.
**摘要**：开发了一种针对线粒体呼吸链复合物亚基的抗体组合，包括NDUFS7抗体。实验验证了该抗体在小鼠和人类组织中的特异性，并通过免疫沉淀证明NDUFS7在复合物I组装中的关键作用，为研究线粒体疾病机制提供工具。
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3. **文献名称**：*NDUFS7 downregulation promotes tumor metastasis via metabolic reprogramming in hepatocellular carcinoma*
**作者**：Chen Y, et al.
**摘要**：利用NDUFS7抗体进行免疫印迹和免疫荧光实验，发现肝癌细胞中NDUFS7表达降低与线粒体代谢异常及转移能力增强相关，提示其作为潜在治疗靶点。
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**备注**：以上文献为示例模板，实际文献需通过PubMed、Web of Science等平台检索关键词“NDUFS7 antibody”、“NDUFS7 AND mitochondrial complex I”获取。若需具体文献，可提供更详细的研究背景或应用场景进一步筛选。
The NDUFS7 antibody is a valuable tool for studying mitochondrial complex I (NADH:ubiquinone oxidoreductase), a critical component of the electron transport chain. NDUFS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 7) is a nuclear-encoded subunit of complex I, responsible for catalyzing the first step of oxidative phosphorylation. This 20 kDa protein contains conserved cysteine residues that coordinate iron-sulfur clusters essential for electron transfer. Mutations in the NDUFS7 gene are associated with mitochondrial disorders, including Leigh syndrome and other neurodegenerative conditions characterized by impaired energy metabolism.
Researchers commonly use NDUFS7 antibodies to investigate complex I assembly, function, and expression in diseases like Parkinson's disease, cancer, and aging-related pathologies. These antibodies enable detection of NDUFS7 through techniques such as Western blotting, immunofluorescence, and immunohistochemistry, helping assess mitochondrial health and complex I deficiencies. Validated antibodies typically show specificity for the ~20 kDa band in mitochondrial lysates and demonstrate reduced signal in NDUFS7-knockout models. Commercial versions are available from multiple suppliers (e.g., Abcam, Santa Cruz Biotechnology), often tested in human, mouse, and rat samples. Proper controls, including mitochondrial enrichment verification and complex I activity assays, are recommended when using this antibody to ensure accurate interpretation in studies of cellular bioenergetics and mitochondrial dysfunction.
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