| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CI-75k; CI-75Kd; PRO1304 |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NDUFS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFS1抗体的3篇参考文献示例(文献信息为虚构示例，仅用于格式演示)：
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1. **文献名称**: "NDUFS1 deficiency in mitochondrial complex I disrupts cellular energy metabolism"
**作者**: Smith J, et al.
**摘要**: 研究利用NDUFS1抗体通过Western blot和免疫组化分析，发现NDUFS1蛋白在线粒体复合物I缺陷患者肌肉组织中的表达显著降低，导致ATP合成障碍及氧化应激增加。
2. **文献名称**: "Antibody-based profiling of mitochondrial proteins in Parkinson's disease models"
**作者**: Lee H, et al.
**摘要**: 该研究使用NDUFS1抗体检测帕金森病小鼠模型的脑组织中线粒体复合物I亚基的表达变化，发现NDUFS1的异常聚集与神经元退行性病变相关。
3. **文献名称**: "Role of NDUFS1 in cancer cell metabolic reprogramming"
**作者**: Chen R, et al.
**摘要**: 通过NDUFS1抗体的免疫沉淀和免疫荧光技术，揭示结直肠癌细胞中NDUFS1的表达上调促进糖酵解向氧化磷酸化转换，影响肿瘤生长。
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(注：以上文献信息为模拟生成，实际引用需查询PubMed、Google Scholar等数据库获取真实数据。)
The NDUFS1 antibody is a crucial tool for studying mitochondrial complex I (NADH:ubiquinone oxidoreductase), a key enzyme in the electron transport chain. NDUFS1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 1) is a core catalytic subunit of complex I, located in the mitochondrial matrix-facing arm. It plays a vital role in electron transfer during oxidative phosphorylation (OXPHOS) and ATP production. Antibodies targeting NDUFS1 are widely used to detect protein expression levels, assess complex I assembly, and investigate mitochondrial dysfunction.
Research involving NDUFS1 antibodies often focuses on diseases linked to complex I deficiencies, such as Leigh syndrome, mitochondrial encephalopathies, and neurodegenerative disorders like Parkinson’s disease. Mutations in the NDUFS1 gene are associated with severe metabolic disorders, making this antibody valuable for diagnostic and mechanistic studies. It is also employed in cancer research, as altered complex I activity correlates with tumor metabolism.
Most NDUFS1 antibodies are raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation typically includes Western blotting, immunofluorescence, and immunohistochemistry, often paired with knockout controls to confirm specificity. Its applications extend to understanding tissue-specific OXPHOS variations and evaluating therapeutic interventions targeting mitochondrial energy pathways. As mitochondrial research expands, the NDUFS1 antibody remains essential for unraveling cellular bioenergetics and disease mechanisms.
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