| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NCF-2; NOXA2; P67PHOX; P67-PHOX |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NCF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于NCF2抗体的参考文献摘要(基于公开文献信息模拟整理，非真实引用)：
1. **文献名称**：*"NCF2/p67phox forms a functional complex with cytochrome b558 in NADPH oxidase activation"*
**作者**：Roos D et al.
**摘要**：本研究利用NCF2特异性抗体，通过免疫共沉淀和Western blot技术，验证了NCF2蛋白与细胞色素b558的相互作用，揭示了NADPH氧化酶复合体在活性氧(ROS)生成中的关键结构基础。
2. **文献名称**：*"Autoantibodies against NCF2 in systemic lupus erythematosus: a novel biomarker for disease activity"*
**作者**：Heyninck K et al.
**摘要**：报道了系统性红斑狼疮(SLE)患者血清中存在的抗NCF2自身抗体，并通过ELISA和免疫荧光实验证明其与疾病活动度相关，提示NCF2抗体可能作为SLE的新型诊断标志物。
3. **文献名称**：*"Targeted disruption of NCF2 reveals its essential role in phagocyte ROS production and bacterial killing"*
**作者**：Matute JD et al.
**摘要**：利用CRISPR/Cas9构建NCF2基因敲除模型，结合NCF2抗体进行蛋白表达验证，证实NCF2缺失导致中性粒细胞ROS生成缺陷，进而削弱宿主对细菌的清除能力。
4. **文献名称**：*"Structural insights into NCF2 mutations causing chronic granulomatous disease"*
**作者**：Bedard K & Krause KH
**摘要**：通过X射线晶体学解析NCF2突变体的三维结构，结合抗体介导的构象分析，揭示了特定突变如何破坏NCF2与p40phox的结合，最终导致慢性肉芽肿病(CGD)的分子机制。
(注：以上内容为模拟生成，实际文献需通过PubMed等数据库检索核实。)
The NCF2 (Neutrophil Cytosolic Factor 2) antibody is primarily used to detect the NCF2 protein, a critical component of the NADPH oxidase complex in phagocytes. NCF2. encoded by the *NCF2* gene on chromosome 1q25. partners with NCF1 and NCF4 to form the cytosolic p67phox subunit. This complex facilitates reactive oxygen species (ROS) production, essential for microbial killing and immune regulation. Mutations in *NCF2* are linked to chronic granulomatous disease (CGD), a rare immunodeficiency characterized by impaired oxidative burst and recurrent infections. Additionally, NCF2 dysfunction has been associated with autoimmune disorders like systemic lupus erythematosus (SLE), where altered ROS signaling may disrupt immune tolerance.
NCF2 antibodies are vital tools in research and diagnostics. They help identify protein expression levels in immune cells, assess genetic mutations via Western blot or immunofluorescence, and explore mechanisms of oxidative stress in inflammatory diseases. Commercial antibodies are often validated for specificity against human or murine NCF2 isoforms. Recent studies also utilize these antibodies to investigate NCF2's role beyond immunity, including its potential involvement in cancer progression or metabolic disorders. By enabling precise detection of NCF2. these antibodies contribute to understanding disease pathogenesis and developing targeted therapies for CGD, autoimmune conditions, and other ROS-related disorders.
×
