| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NSBP1; NBP-45 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HMGN5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGN5抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*HMGN5 promotes cancer cell invasion and epithelial-mesenchymal transition*
**作者**：Zhang Y, Wang X, et al.
**摘要**：该研究利用HMGN5特异性抗体，发现其在乳腺癌细胞中高表达，并通过调控基质金属蛋白酶(MMPs)促进肿瘤侵袭和转移，提示HMGN5可能成为癌症治疗的靶点。
2. **文献名称**：*The role of HMGN5 in chromatin remodeling and transcriptional regulation*
**作者**：Furusawa T, Ko JH, et al.
**摘要**：通过免疫沉淀和染色质分析技术，研究发现HMGN5抗体可用于检测其与染色质的相互作用，揭示HMGN5通过松解染色质结构增强特定基因(如癌基因)的转录活性。
3. **文献名称**：*HMGN5 interacts with androgen receptor to drive prostate cancer progression*
**作者**：Li H, Gupta S, et al.
**摘要**：该文献使用HMGN5抗体进行Western blot和免疫组化，证实HMGN5在前列腺癌细胞中与雄激素受体(AR)结合，促进细胞增殖和耐药性，为去势抵抗性前列腺癌提供了机制解释。
HMGN5 antibody is a research tool designed to detect and study the High Mobility Group Nucleosome-binding protein 5 (HMGN5), a member of the HMGN family. HMGN proteins are chromatin-associated factors that regulate chromatin structure and gene expression by interacting with nucleosomes. HMGN5. also known as NSBP1 (Nucleosome-binding protein 1), is distinguished by its ability to bind nucleosomes and modulate chromatin accessibility, influencing DNA replication, repair, and transcriptional activity. It is ubiquitously expressed but shows elevated levels in certain cancers, including prostate, breast, and ovarian cancers, where it is implicated in promoting cell proliferation, invasion, and metastasis through interactions with pathways like Wnt/β-catenin or EGFR signaling.
HMGN5 antibodies are widely used in molecular and cellular biology to investigate the protein's expression patterns, subcellular localization, and functional mechanisms. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP), aiding in the study of HMGN5's role in epigenetic regulation and disease progression. Researchers also utilize HMGN5 antibodies to explore its potential as a therapeutic target or biomarker in cancer, leveraging experimental models like cell lines or xenografts. Validation of antibody specificity (e.g., via knockout controls) is critical to ensure accurate detection, given structural similarities among HMGN family members.
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