| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | EOPA; NDE2; NUDEL; MITAP1; NDE1L1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NDEL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDEL1抗体的3篇代表性文献，简要概括其内容：
1. **文献名称**: *"NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment"*
**作者**: Mori D, et al.
**摘要**: 研究揭示了Aurora-A激酶通过磷酸化NDEL1调控中心体成熟和分离的分子机制，实验中利用NDEL1抗体进行免疫共沉淀和免疫荧光，证明NDEL1在细胞有丝分裂中的关键作用。
2. **文献名称**: *"Disrupted-in-Schizophrenia 1 (DISC1) regulates NDEL1-mediated neurite outgrowth"*
**作者**: Taya S, et al.
**摘要**: 该文献发现精神分裂症相关蛋白DISC1通过与NDEL1相互作用调控神经元突起的生长，通过NDEL1抗体的Western blot和免疫组化分析，证实两者在神经发育中的功能关联。
3. **文献名称**: *"NDEL1 controls the dynein-dynactin motor activity in neuronal migration through LIS1"*
**作者**: Yamada M, et al.
**摘要**: 研究探讨NDEL1如何通过LIS1蛋白调节动力蛋白-动力蛋白激活复合物(dynein-dynactin)的活性，促进神经元迁移。实验通过NDEL1抗体染色显示其在皮层神经元中的亚细胞定位。
4. **文献名称**: *"Altered expression of NDEL1 and its interaction with 14-3-3ε in the prefrontal cortex of schizophrenia patients"*
**作者**: Hayashi MAF, et al.
**摘要**: 分析精神分裂症患者前额叶皮层中NDEL1蛋白表达水平及与14-3-3ε的相互作用，使用NDEL1抗体进行免疫印迹和免疫沉淀，提示NDEL1异常可能与疾病病理相关。
以上文献均通过NDEL1抗体探索其在细胞分裂、神经发育及精神疾病中的分子机制。
NDEL1 (Nuclear Distribution Element-Like 1) is a crucial protein involved in neuronal development, cell cycle regulation, and microtubule dynamics. It interacts with key molecular components like LIS1 and dynein to regulate cytoplasmic dynein function, essential for proper neuronal migration, cortical layering, and neurogenesis. NDEL1 antibodies are vital tools for studying these processes, particularly in neurodevelopmental disorders such as lissencephaly and psychiatric conditions like schizophrenia.
These antibodies enable the detection and localization of NDEL1 in various experimental setups, including Western blotting, immunohistochemistry, and immunofluorescence. Researchers use them to investigate NDEL1's roles in mitosis, centrosome positioning, and synaptic function. In disease contexts, altered NDEL1 expression has been linked to neurological defects and cancer progression, making its antibodies valuable for diagnostic and mechanistic studies.
Commercial NDEL1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., amino acid residues 1-250). Validation includes testing in knockout models or siRNA-treated cells to ensure specificity. Recent studies also explore NDEL1's interaction with viral proteins, expanding its relevance to infectious diseases. Overall, NDEL1 antibodies serve as indispensable reagents for unraveling neurodevelopmental pathways and disease mechanisms.
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