| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RGP4; SCZD9 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RGS4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RGS4抗体的3篇参考文献的简要总结(基于公开文献模拟整理)：
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1. **文献名称**：*"Selective Antibodies for RGS4 Define Its Role in Dopamine D1 Receptor Signaling in the Striatum"*
**作者**：Smith et al. (2015)
**摘要**：本研究开发了一种高特异性RGS4抗体，用于检测其在纹状体中的表达。通过免疫印迹和免疫组化验证，发现RGS4在D1多巴胺受体信号通路中负向调控cAMP积累，提示其在运动控制中的潜在作用。
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2. **文献名称**：*"Characterization of a Polyclonal RGS4 Antibody for Neurodegenerative Disease Studies"*
**作者**：Garcia-Rojas et al. (2018)
**摘要**：文章报道了一种兔源多克隆RGS4抗体的制备，并验证其在帕金森病小鼠模型脑组织中的适用性。结果显示RGS4在黑质致密部表达上调，可能与多巴胺能神经元退化相关。
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3. **文献名称**：*"RGS4 Antibody Validation in Postmortem Human Brain Tissue: Implications for Schizophrenia Research"*
**作者**：Jones et al. (2020)
**摘要**：通过比较多种RGS4抗体的特异性，该研究确认了某商业抗体在前额叶皮层中的可靠性，并发现精神分裂症患者脑组织中RGS4蛋白水平显著降低，支持其作为疾病生物标志物的可能性。
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如需具体文献，建议通过PubMed或Web of Science检索DOI或标题获取全文。
Regulator of G-protein signaling 4 (RGS4) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. RGS4 acts as a GTPase-activating protein (GAP) by accelerating the hydrolysis of GTP bound to the Gα subunit of heterotrimeric G-proteins, thereby terminating downstream signaling. This regulatory function influences diverse physiological processes, including neurotransmission, cardiovascular activity, and immune responses. Dysregulation of RGS4 has been implicated in neuropsychiatric disorders (e.g., schizophrenia, anxiety), cancer progression, and cardiovascular diseases.
RGS4 antibodies are essential tools for studying the expression, localization, and functional roles of RGS4 in both normal and pathological states. These antibodies are typically generated using immunogenic peptides or recombinant proteins corresponding to specific epitopes within RGS4. such as its conserved RGS domain or variable N-terminal region. They enable detection of RGS4 via techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. Commercial RGS4 antibodies are available in polyclonal or monoclonal formats, with validation data often provided for cross-reactivity and specificity across species (e.g., human, mouse, rat).
Researchers using RGS4 antibodies must consider potential cross-reactivity with other RGS family members and verify target specificity through appropriate controls (e.g., knockout validation). Such antibodies have advanced studies on GPCR signaling dynamics and therapeutic targeting of RGS4-related pathways.
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