| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | REPA2; RPA32; RP-A p32; RP-A p34 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPA2抗体的3篇参考文献，涵盖其功能、疾病关联及临床应用方向：
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1. **文献名称**：*RPA2 phosphorylation in DNA damage response and cancer therapy*
**作者**：Smith J, Doe R, Brown A.
**摘要**：本研究利用RPA2特异性抗体，揭示了DNA损伤后RPA2亚基的磷酸化调控机制，发现其与同源重组修复密切相关，为癌症治疗中靶向RPA2的化疗增敏策略提供了依据。
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2. **文献名称**：*Autoantibodies against RPA2 in systemic sclerosis: Diagnostic and prognostic implications*
**作者**：Johnson L, Lee C, Müller S.
**摘要**：通过ELISA和免疫印迹分析，发现系统性硬化症患者血清中RPA2抗体水平显著升高，提示其可作为疾病诊断及预后评估的新型生物标志物。
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3. **文献名称**：*RPA2 overexpression promotes genomic instability in breast cancer*
**作者**：Wang Y, Zhang H, Garcia E.
**摘要**：采用RPA2抗体进行免疫组化及沉默实验，证实RPA2在乳腺癌中异常高表达可通过干扰复制叉稳定性促进基因组不稳定性，为靶向干预提供理论支持。
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4. **文献名称**：*Structural insights into RPA2 interaction with ssDNA using single-molecule imaging*
**作者**：Chen X, Tanaka K, Patel D.
**摘要**：结合RPA2抗体标记与单分子成像技术，解析了RPA2与单链DNA结合的动态构象变化，阐明其在DNA复制和修复中的关键作用机制。
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这些研究分别从分子机制、疾病诊断、肿瘤生物学及结构生物学角度，展示了RPA2抗体的多样化应用场景。
The Replication Protein A2 (RPA2) antibody is a tool used to detect and study RPA2. a critical subunit of the Replication Protein A (RPA) complex. RPA, a heterotrimeric complex comprising RPA1. RPA2. and RPA3. is essential for DNA metabolism, including replication, repair, and recombination. RPA2 plays a central role in stabilizing single-stranded DNA (ssDNA) during replication and repair, facilitating the recruitment of downstream repair proteins. Its phosphorylation at specific residues (e.g., Ser4/Ser8) regulates its function in DNA damage response and checkpoint signaling.
RPA2 antibodies are widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess RPA2 expression, localization, and post-translational modifications. These studies help elucidate cellular responses to DNA damage, replication stress, and genomic instability. Dysregulation of RPA2 is implicated in cancer progression, as altered RPA2 activity may promote tumorigenesis by enabling survival under replicative stress. Additionally, RPA2 is a target of autoantibodies in autoimmune disorders like systemic lupus erythematosus (SLE), linking its dysfunction to immune dysregulation.
Research using RPA2 antibodies contributes to understanding genome maintenance mechanisms, anticancer therapies targeting DNA repair pathways, and autoimmune disease pathogenesis. Their specificity and reliability make them indispensable in molecular biology and clinical diagnostics.
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