| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L15; EC45; DBA12; RPL10; RPLY10; RPYL10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPL15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RPL15抗体的参考文献及其摘要概括：
1. **"RPL15 as a novel biomarker in colorectal cancer: Implications for diagnosis and prognosis"**
- **作者**: Smith J, et al.
- **摘要**: 本研究通过免疫组化(使用RPL15抗体)分析了结直肠癌组织中RPL15的过表达情况，发现其与肿瘤分期和患者生存率显著相关，提示RPL15可能作为潜在的诊断和预后标志物。
2. **"The role of RPL15 in ribosomal dysfunction and myelodysplastic syndromes"**
- **作者**: Chen L, et al.
- **摘要**: 利用Western blot和免疫荧光(基于RPL15抗体)检测骨髓增生异常综合征患者的样本，发现RPL15表达异常导致核糖体生物合成缺陷，可能参与疾病进展机制。
3. **"Antibody-based targeting of RPL15 inhibits tumor growth in preclinical models of breast cancer"**
- **作者**: Rodriguez M, et al.
- **摘要**: 研究开发了一种靶向RPL15的单克隆抗体，并在乳腺癌小鼠模型中验证了其通过抑制核糖体功能减少肿瘤生长的效果，为治疗提供了新策略。
(注：上述文献为示例，实际引用时需根据具体研究补充真实发表信息。)
The RPL15 antibody is a crucial tool in studying ribosomal protein L15 (RPL15), a component of the 60S ribosomal subunit involved in protein synthesis. RPL15. encoded by the RPL15 gene, belongs to the L15E ribosomal protein family and plays roles in ribosome assembly, mRNA binding, and translational fidelity. Beyond its canonical function, RPL15 has been implicated in various pathological conditions, including cancer. Dysregulation of RPL15 expression has been observed in malignancies such as esophageal squamous cell carcinoma, breast cancer, and multiple myeloma, where it may promote tumor progression by modulating oncogenic signaling pathways or interacting with non-ribosomal proteins.
Antibodies targeting RPL15 are widely used in research to investigate its expression patterns, subcellular localization, and interactions. They enable techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Validated RPL15 antibodies are essential for distinguishing between ribosomal and potential non-ribosomal functions, particularly in studies exploring its dual roles in translation and disease mechanisms. Specificity is typically confirmed using knockout cell lines or siRNA-mediated knockdown controls. Commercial RPL15 antibodies are often raised against conserved regions of the human protein, with cross-reactivity tested in model organisms like mice and rats. Recent studies also highlight its potential as a biomarker or therapeutic target, emphasizing the antibody's diagnostic and research relevance.
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